Expression vectors for human α-interferon (Hu-IFN-α) J1, a site-specific mutant [Ser116]Hu-IFN-αJ1, and Hu-IFN-αJ/C or Hu-IFN-αC/ J hybrids were constructed and expressed in Escherichia coli. These interferons and others were purified by immunoaffinity chromatography with a monoclonal antibody against human α-interferon. Their antiviral activity and ability to stimulate natural killer cell activity were determined in comparison to several other human interferons. These results provide some insight into structure-activity relationships for stimulating natural killer cells and confirm our previous conclusions that antiviral activity cannot be used to predict other activities for an individual IFN-α species. The observations suggest that the tertiary structure rather than any specific linear sequence of amino acids regulates the ability of the interferons to stimulate natural killer cell activity.
|Number of pages||5|
|Publication status||Published - 1990 Sep 1|
All Science Journal Classification (ASJC) codes
- Cancer Research