α-Interferon Structure and Natural Killer Cell Stimulatory Activity

Bo Liang Li, Xiao Xia Zhao, Xin Yuan Liu, Hyon Suk Kim, Karel Raska, John R. Ortaldo, Barbara Schwartz, Sidney Pestka

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Expression vectors for human α-interferon (Hu-IFN-α) J1, a site-specific mutant [Ser116]Hu-IFN-αJ1, and Hu-IFN-αJ/C or Hu-IFN-αC/ J hybrids were constructed and expressed in Escherichia coli. These interferons and others were purified by immunoaffinity chromatography with a monoclonal antibody against human α-interferon. Their antiviral activity and ability to stimulate natural killer cell activity were determined in comparison to several other human interferons. These results provide some insight into structure-activity relationships for stimulating natural killer cells and confirm our previous conclusions that antiviral activity cannot be used to predict other activities for an individual IFN-α species. The observations suggest that the tertiary structure rather than any specific linear sequence of amino acids regulates the ability of the interferons to stimulate natural killer cell activity.

Original languageEnglish
Pages (from-to)5328-5332
Number of pages5
JournalCancer Research
Volume50
Issue number17
Publication statusPublished - 1990 Sep 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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