Chiral monolithic capillary columns for reversed-phase capillary electrochromatography (CEC) and for nano-HPLC were prepared by linking β-cyclodextrin modifier into the acrylic monolithic phase. Columns with the physically and chemically bonded β-cyclodextrin derivatives were tested under CEC and nano-HPLC conditions; enatioselective separation of (-)-ephedrine/(+)-pseudoephedrine and (+/-)-ibuprofen was successfully performed. The separation efficiency of CEC and HPLC was examined and compared; resolution of (+/-) ibuprophen was 2.45 and 2.97 for CEC and HPLC respectively, number theoretical plates of thiourea were 41,600 N/m in CEC.
|Number of pages||12|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - 2002 Oct 12|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry