β-cyclodextrin-modified monolithic stationary phases for capillary electrochromatography and nano-HPLC chiral analysis of ephedrine and ibuprofen

Martin Pumera, Ivan Jelinek, Jindrich Jindrich, Oldrich Benada

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Chiral monolithic capillary columns for reversed-phase capillary electrochromatography (CEC) and for nano-HPLC were prepared by linking β-cyclodextrin modifier into the acrylic monolithic phase. Columns with the physically and chemically bonded β-cyclodextrin derivatives were tested under CEC and nano-HPLC conditions; enatioselective separation of (-)-ephedrine/(+)-pseudoephedrine and (+/-)-ibuprofen was successfully performed. The separation efficiency of CEC and HPLC was examined and compared; resolution of (+/-) ibuprophen was 2.45 and 2.97 for CEC and HPLC respectively, number theoretical plates of thiourea were 41,600 N/m in CEC.

Original languageEnglish
Pages (from-to)2473-2484
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume25
Issue number16
DOIs
Publication statusPublished - 2002 Oct 12

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Capillary Electrochromatography
Ephedrine
Ibuprofen
Cyclodextrins
High Pressure Liquid Chromatography
Pseudoephedrine
Thiourea
Acrylics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this

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β-cyclodextrin-modified monolithic stationary phases for capillary electrochromatography and nano-HPLC chiral analysis of ephedrine and ibuprofen. / Pumera, Martin; Jelinek, Ivan; Jindrich, Jindrich; Benada, Oldrich.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 25, No. 16, 12.10.2002, p. 2473-2484.

Research output: Contribution to journalArticle

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AU - Pumera, Martin

AU - Jelinek, Ivan

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AU - Benada, Oldrich

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