β-Glycosidase-assisted bioconversion of ginsenosides in purified crude saponin and extracts from red ginseng (Panax ginseng C. A. Meyer)

Hae Ryung Park, Kashif Ghafoor, Dohyun Lee, Sunghan Kim, Sun Hyoung Kim, Jiyong Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Major ginsenosides in ginseng (Panax ginseng) and its products are highly glycosylated, hence poorly absorbed in the gastrointestinal tract. β-Glycosidase-assisted deglycosylation of pure ginsenosides was peformed to study bioconversion mechanisms. Ginsenoside standard compounds, crude saponin, and red ginseng extracts were incubated with β-glycosidase (0.05% w/v, 55°C). β-Glycosidase has a broad specificity for β-glycosidic bonds, hydrolyzing the β-(1→6), α-(1→6), and α-(1→2) glycosidic linkages. The final metabolite of protopanaxadiol ginsenosides was Rg3 while the metabolite of protopanaxatriol ginsenosides was Rh1. β-Glycosidase treatment of red ginseng extracts resulted in a decrease in the amounts of Rb1, Rc, Re, and Rg2 after 24 h, whereas levels of the less glycosylated Rd, Rb1, Rg, Rg3, Rg1, and Rh1 forms increased. When crude saponin was incubated with β-glycosidase for 24 h, levels of Rb1, Rc, Re, and Rg1 decreased while levels of Rd, Rg3, and Rh1 increased as deglycosylated ginsenosides.

Original languageEnglish
Pages (from-to)1629-1638
Number of pages10
JournalFood Science and Biotechnology
Volume22
Issue number6
DOIs
Publication statusPublished - 2013 Dec 30

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Ginsenosides
Panax ginseng
Panax
glycosidases
Glycoside Hydrolases
Saponins
biotransformation
Complex Mixtures
saponins
extracts
glycosidic linkages
metabolites
Gastrointestinal Tract
gastrointestinal system
ginsenosides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Park, Hae Ryung ; Ghafoor, Kashif ; Lee, Dohyun ; Kim, Sunghan ; Kim, Sun Hyoung ; Park, Jiyong. / β-Glycosidase-assisted bioconversion of ginsenosides in purified crude saponin and extracts from red ginseng (Panax ginseng C. A. Meyer). In: Food Science and Biotechnology. 2013 ; Vol. 22, No. 6. pp. 1629-1638.
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β-Glycosidase-assisted bioconversion of ginsenosides in purified crude saponin and extracts from red ginseng (Panax ginseng C. A. Meyer). / Park, Hae Ryung; Ghafoor, Kashif; Lee, Dohyun; Kim, Sunghan; Kim, Sun Hyoung; Park, Jiyong.

In: Food Science and Biotechnology, Vol. 22, No. 6, 30.12.2013, p. 1629-1638.

Research output: Contribution to journalArticle

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AU - Park, Hae Ryung

AU - Ghafoor, Kashif

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AU - Kim, Sunghan

AU - Kim, Sun Hyoung

AU - Park, Jiyong

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