ω-Transaminase-catalyzed kinetic resolution of chiral amines using l-threonine as an amino acceptor precursor

M. Shaheer Malik, Eul Soo Park, Jong Shik Shin

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Abstract

Kinetic resolution of chiral amines using l-threonine as a cosubstrate was demonstrated by a biocatalytic strategy in which (S)-selective ω-transaminase (ω-TA) was coupled with threonine deaminase (TD), eliminating the need to use an expensive keto acid as an amino acceptor. The coupled enzyme reaction enabled simultaneous production of enantiopure (R)-amine and l-homoalanine which are pharmaceutically important building blocks. To extend the versatility of this strategy to production of both enantiomers of chiral amines, (R)-selective ω-TA coupled with TD was employed to produce (S)-amine.

Original languageEnglish
Pages (from-to)2137-2140
Number of pages4
JournalGreen Chemistry
Volume14
Issue number8
DOIs
Publication statusPublished - 2012 Aug

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All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution

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