1T-Phase WS2 Protein-Based Biosensor

Rou Jun Toh, Carmen C. Mayorga-Martinez, Zdenek Sofer, Martin Pumera

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


Metallic 1T-phase transition metal dichalcogenides have been recognized for their desirable properties like high surface-to-volume ratio, high conductivity, and capacitive behavior, making them outstanding for catalytic and sensing applications. Herein, a hydrogen peroxide (H2O2) biosensor is constructed by the immobilization of hemoglobin (Hb) on 1T-phase WS2 (1T-WS2) sheets, and entrapment by glutaraldehyde. 1T-WS2 not only displays electrocatalytic activity toward the reduction of H2O2 but also provides a high surface-to-volume ratio and conductive platform for the immobilization of Hb and facilitation of its electron transfer to the electrode surface. The advantageous role of 1T-phase WS2 is further demonstrated for the construction of a heme-based H2O2 biosensor compared to its 1T-phase MoS2, MoSe2, and WSe2 counterparts. Synergistic interactions between 1T-WS2 and Hb result in a H2O2 biosensor with high analytical performance in terms of wide range, sensitivity, selectivity, reproducibility, repeatability, and stability. These findings have profound impact in the research fields of electrochemical sensing and biodiagnostics.

Original languageEnglish
Article number1604923
JournalAdvanced Functional Materials
Issue number5
Publication statusPublished - 2017 Feb 3

Bibliographical note

Funding Information:
M.P. acknowledges a Tier 2 grant (MOE2013-T2-1-056; ARC 35/13) from the Ministry of Education, Singapore. R.J.T. acknowledges financial support from the National Research Foundation (NRF), Prime Minister's Office, Singapore, under the CREATE programme, Singapore-MIT Alliance for Research and Technology (SMART) BioSystems and Micromechanics (BioSyM) IRG. Z.S. was supported by Czech Science Foundation (GACR No. 16-05167S).

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics


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