20(S)-Ginsenoside Rg3 prevents endothelial cell apoptosis via inhibition of a mitochondrial caspase pathway

Jeong Ki Min, Jung Ho Kim, Young Lai Cho, Yong Sun Maeng, Shin Jeong Lee, Bo Jeong Pyun, Young Myeong Kim, Jeong Hill Park, Young-Guen Kwon

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Ginseng, refering to the roots of the species of the genus Panax ginseng, has been widely used in traditional oriental medicine for its wide spectrum of medicinal effects, such as anti-inflammatory, anti-tumorigenic, adaptogenic, and anti-aging activities. Many of its medicinal effects are attributed to the triterpene glycosides known as ginsenosides. In this study, we report a novel anti-apoptotic activity of 20(S)-ginsenoside Rg3 ((20S)Rg3) and its underlying molecular mechanism in human endothelial cells (ECs). ECs undergo apoptosis associated with increased LEHDase (caspase-9) and DEVDase (caspase-3) activity and DNA fragmentation after 24 h of serum deprivation. These apoptotic markers were suppressed by the addition of (20S)Rg3. (20S)Rg3 increased the expression of Bax and conversely decreased Bcl-2. (20S)Rg3 potently induced a rapid and sustained Akt activation and Bad phosphorylation, resulting in the inhibition of mitochondrial cytochrome c release. These anti-apoptotic activities of (20S)Rg3 were significantly abrogated in cells expressing dominant negative Akt. Taken together, our results suggest that (20S)Rg3 prevents EC apoptosis via Akt-dependent inhibition of the mitochondrial apoptotic signaling pathway. The novel property of (20S)Rg3 may be valuable for developing new pharmaceutical means that will control unwanted endothelial cell death at the site of vascular injury.

Original languageEnglish
Pages (from-to)987-994
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume349
Issue number3
DOIs
Publication statusPublished - 2006 Oct 27

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Endothelial cells
Caspases
Endothelial Cells
Apoptosis
Panax
East Asian Traditional Medicine
Ginsenosides
Triterpenes
Phosphorylation
Caspase 9
Vascular System Injuries
DNA Fragmentation
Cell death
Glycosides
Cytochromes c
Caspase 3
Medicine
Cell Death
Anti-Inflammatory Agents
Aging of materials

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Min, Jeong Ki ; Kim, Jung Ho ; Cho, Young Lai ; Maeng, Yong Sun ; Lee, Shin Jeong ; Pyun, Bo Jeong ; Kim, Young Myeong ; Park, Jeong Hill ; Kwon, Young-Guen. / 20(S)-Ginsenoside Rg3 prevents endothelial cell apoptosis via inhibition of a mitochondrial caspase pathway. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 349, No. 3. pp. 987-994.
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20(S)-Ginsenoside Rg3 prevents endothelial cell apoptosis via inhibition of a mitochondrial caspase pathway. / Min, Jeong Ki; Kim, Jung Ho; Cho, Young Lai; Maeng, Yong Sun; Lee, Shin Jeong; Pyun, Bo Jeong; Kim, Young Myeong; Park, Jeong Hill; Kwon, Young-Guen.

In: Biochemical and Biophysical Research Communications, Vol. 349, No. 3, 27.10.2006, p. 987-994.

Research output: Contribution to journalArticle

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