3,3'-diethylthiatricarbocyanine iodide

A highly sensitive chiroptical reporter of DNA helicity and sequence

Jung Kyu Choi, Alessandro D'Urso, Mitch Trauernicht, Murtaza Shabbir-Hussain, Andrea E. Holmes, Milan Balaz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.

Original languageEnglish
Pages (from-to)8052-8062
Number of pages11
JournalInternational journal of molecular sciences
Volume12
Issue number11
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Z-Form DNA
Methenamine
B-Form DNA
Circular dichroism spectroscopy
Polynucleotides
Spermine
DNA sequences
Dichroism
Iodides
Circular Dichroism
iodides
DNA
deoxyribonucleic acid
dichroism
polynucleotides
Spectrum Analysis
signatures
spectroscopy
3,3'-diethylthiatricarbocyanine
poly(dC-dG)

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Choi, Jung Kyu ; D'Urso, Alessandro ; Trauernicht, Mitch ; Shabbir-Hussain, Murtaza ; Holmes, Andrea E. ; Balaz, Milan. / 3,3'-diethylthiatricarbocyanine iodide : A highly sensitive chiroptical reporter of DNA helicity and sequence. In: International journal of molecular sciences. 2011 ; Vol. 12, No. 11. pp. 8052-8062.
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3,3'-diethylthiatricarbocyanine iodide : A highly sensitive chiroptical reporter of DNA helicity and sequence. / Choi, Jung Kyu; D'Urso, Alessandro; Trauernicht, Mitch; Shabbir-Hussain, Murtaza; Holmes, Andrea E.; Balaz, Milan.

In: International journal of molecular sciences, Vol. 12, No. 11, 01.11.2011, p. 8052-8062.

Research output: Contribution to journalArticle

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AU - Choi, Jung Kyu

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