Digital in-line holographic microscopy (DIHM) has attracted attention because of its simple but powerful three-dimensional (3D) imaging capability. To improve the spatial resolution, 3D image reconstruction algorithms use numerical magnification, which generates distortions in the generated images. We propose a method to overcome this problem by using the simple relation between the object and image positions in 3D space. Several holograms were taken while translating a resolution target at different axial positions by a motorized stage. We demonstrated the effectiveness of our method by reconstructing the 3D positions of 3-μm-diameter polymer beads on a tilted slide glass from a single measured hologram.
Bibliographical noteFunding Information:
This work was financially supported by the MEST through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (2017R1A2B4003950), the Basic Science Research Program through the National Research Foundation of Korea (NRF-2013R1A1A2062448), the Center for Advanced Meta-Materials (CAMM) funded by the Ministry of Science, ICT and Future Planning as a Global Frontier Project (CAMM-2014M3A6B3063712), the Technology Innovation Program (10062417) funded by the Ministry of Trade, Industry and Energy (MI), and the Ministry of Education, Science and Technology of Korea through the BK21 program.
© 2017 Optical Society of America.
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics