3D image distortion problem in digital in-line holographic microscopy and its effective solution

Heejung Lee, Philjun Jeon, Dug Young Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Digital in-line holographic microscopy (DIHM) has attracted attention because of its simple but powerful three-dimensional (3D) imaging capability. To improve the spatial resolution, 3D image reconstruction algorithms use numerical magnification, which generates distortions in the generated images. We propose a method to overcome this problem by using the simple relation between the object and image positions in 3D space. Several holograms were taken while translating a resolution target at different axial positions by a motorized stage. We demonstrated the effectiveness of our method by reconstructing the 3D positions of 3-μm-diameter polymer beads on a tilted slide glass from a single measured hologram.

Original languageEnglish
Pages (from-to)21969-21980
Number of pages12
JournalOptics Express
Volume25
Issue number18
DOIs
Publication statusPublished - 2017 Sep 4

Fingerprint

microscopy
translating
image reconstruction
magnification
chutes
beads
spatial resolution
glass
polymers

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

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3D image distortion problem in digital in-line holographic microscopy and its effective solution. / Lee, Heejung; Jeon, Philjun; Kim, Dug Young.

In: Optics Express, Vol. 25, No. 18, 04.09.2017, p. 21969-21980.

Research output: Contribution to journalArticle

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