Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., μLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. In addition, chronic silicon neural probes are practically single implant devices due to the current low success rate of probe recovery. To successfully recover silicon probes, improve upon the quality of electrophysiological recording, and make silicon probe recordings more accessible, we have designed a miniature, low cost, and recoverable microdrive system. The addition of a novel 3D-printed skull baseplate makes the surgery less invasive, faster, and simpler for both rats and mice. We provide detailed procedural instructions and print designs, allowing researchers to adapt and flexibly customize our designs to their experimental usage.
|Publication status||Published - 2021 Aug 20|
Bibliographical noteFunding Information:
We would like to thank our early adaptors in Royer, Diba, and Buzsaki Labs for testing the microdrive and the baseplate and for providing feedback. We also thank Dmitry Rinberg for advice and feedback on designs. This work was supported by U19 NS107616, U19 NS104590, R01 MH122391, NeuroNex MINT (NSF 1707316), and The Lundbeck Foundation. The baseplate was developed for Petersen and Buzsáki (2020) and Vöröslakos et al. (2020), and the microdrives were developed for Vöröslakos et al. (2020).
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Plant Science