3d-printed recoverable microdrive and base plate system for rodent electrophysiology

Mihály Vöröslakos; Hiroyuki Miyawaki; Sebastien Royer; Kamran Diba; Euisik Yoon; Peter C. Petersen; György Buzsáki

doi:10.21769/BioProtoc.4137

3d-printed recoverable microdrive and base plate system for rodent electrophysiology

Mihály Vöröslakos, Hiroyuki Miyawaki, Sebastien Royer, Kamran Diba, Euisik Yoon, Peter C. Petersen, György Buzsáki

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., μLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. In addition, chronic silicon neural probes are practically single implant devices due to the current low success rate of probe recovery. To successfully recover silicon probes, improve upon the quality of electrophysiological recording, and make silicon probe recordings more accessible, we have designed a miniature, low cost, and recoverable microdrive system. The addition of a novel 3D-printed skull baseplate makes the surgery less invasive, faster, and simpler for both rats and mice. We provide detailed procedural instructions and print designs, allowing researchers to adapt and flexibly customize our designs to their experimental usage.

Original language	English
Article number	e4137
Journal	Bio-protocol
Volume	11
Issue number	16
DOIs	https://doi.org/10.21769/BioProtoc.4137
Publication status	Published - 2021 Aug 20

Bibliographical note

Funding Information:
We would like to thank our early adaptors in Royer, Diba, and Buzsaki Labs for testing the microdrive and the baseplate and for providing feedback. We also thank Dmitry Rinberg for advice and feedback on designs. This work was supported by U19 NS107616, U19 NS104590, R01 MH122391, NeuroNex MINT (NSF 1707316), and The Lundbeck Foundation. The baseplate was developed for Petersen and Buzsáki (2020) and Vöröslakos et al. (2020), and the microdrives were developed for Vöröslakos et al. (2020).

Publisher Copyright:
© 2021 Seniko studio Ltd. All rights reserved.

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Plant Science

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10.21769/BioProtoc.4137

Cite this

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title = "3d-printed recoverable microdrive and base plate system for rodent electrophysiology",

abstract = "Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., μLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. In addition, chronic silicon neural probes are practically single implant devices due to the current low success rate of probe recovery. To successfully recover silicon probes, improve upon the quality of electrophysiological recording, and make silicon probe recordings more accessible, we have designed a miniature, low cost, and recoverable microdrive system. The addition of a novel 3D-printed skull baseplate makes the surgery less invasive, faster, and simpler for both rats and mice. We provide detailed procedural instructions and print designs, allowing researchers to adapt and flexibly customize our designs to their experimental usage.",

author = "Mih{\'a}ly V{\"o}r{\"o}slakos and Hiroyuki Miyawaki and Sebastien Royer and Kamran Diba and Euisik Yoon and Petersen, {Peter C.} and Gy{\"o}rgy Buzs{\'a}ki",

note = "Funding Information: We would like to thank our early adaptors in Royer, Diba, and Buzsaki Labs for testing the microdrive and the baseplate and for providing feedback. We also thank Dmitry Rinberg for advice and feedback on designs. This work was supported by U19 NS107616, U19 NS104590, R01 MH122391, NeuroNex MINT (NSF 1707316), and The Lundbeck Foundation. The baseplate was developed for Petersen and Buzs{\'a}ki (2020) and V{\"o}r{\"o}slakos et al. (2020), and the microdrives were developed for V{\"o}r{\"o}slakos et al. (2020). Publisher Copyright: {\textcopyright} 2021 Seniko studio Ltd. All rights reserved.",

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AU - Diba, Kamran

AU - Yoon, Euisik

AU - Petersen, Peter C.

AU - Buzsáki, György

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N2 - Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., μLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. In addition, chronic silicon neural probes are practically single implant devices due to the current low success rate of probe recovery. To successfully recover silicon probes, improve upon the quality of electrophysiological recording, and make silicon probe recordings more accessible, we have designed a miniature, low cost, and recoverable microdrive system. The addition of a novel 3D-printed skull baseplate makes the surgery less invasive, faster, and simpler for both rats and mice. We provide detailed procedural instructions and print designs, allowing researchers to adapt and flexibly customize our designs to their experimental usage.

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3d-printed recoverable microdrive and base plate system for rodent electrophysiology

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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