3D-Printed SARS-CoV-2 RNA Genosensing Microfluidic System

Agustín G. Crevillen, Carmen C. Mayorga-Martinez, Jayraj V. Vaghasiya, Martin Pumera

Research output: Contribution to journalArticlepeer-review

Abstract

Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.

Original languageEnglish
JournalAdvanced Materials Technologies
DOIs
Publication statusAccepted/In press - 2022

Bibliographical note

Funding Information:
This work was supported by the project Advanced Functional Nanorobots (reg. No. CZ.02.1.01/0.0/0.0/15_003/0000444 financed by the EFRR), by Ministry of Education, Youth and Sports (Czech Republic) grant LL2002 under ERC CZ program and the Ministry of Health of the Czech Republic (NU21‐08‐00407). A.G.C. acknowledges the fellowship from Jose Castillejo program (Spain).

Publisher Copyright:
© 2022 Wiley-VCH GmbH

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Industrial and Manufacturing Engineering

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