4-Chlorophenol biodegradation facilitator composed of recombinant multi-biocatalysts immobilized onto montmorillonite

Oh Sung Kwean, Su Yeon Cho, Jun Won Yang, Wooyoun Cho, Sungyoon Park, Yejee Lim, Min Chul Shin, Han Suk Kim, Joonhong Park, Han S. Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A biodegradation facilitator which catalyzes the initial steps of 4-chlorophenol (4-CP) oxidation was prepared by immobilizing multiple enzymes (monooxygenase, CphC-I and dioxygenase, CphA-I) onto a natural inorganic support. The enzymes were obtained via overexpression and purification after cloning the corresponding genes (cphC-I and cphA-I) from Arthrobacter chlorophenolicus A6. Then, the recombinant CphC-I was immobilized onto fulvic acid-activated montmorillonite. The immobilization yield was 60%, and the high enzyme activity (82.6%) was retained after immobilization. Kinetic analysis indicated that the Michaelis-Menten model parameters for the immobilized CphC-I were similar to those for the free enzyme. The enzyme stability was markedly enhanced after immobilization. The immobilized enzyme exhibited a high level of activity even after repetitive use (84.7%) and powdering (65.8%). 4-CP was sequentially oxidized by a multiple enzyme complex, comprising the immobilized CphC-I and CphA-I, via the hydroquinone pathway: oxidative transformation of 4-CP to hydroxyquinol followed by ring fission of hydroxyquinol.

Original languageEnglish
Pages (from-to)268-275
Number of pages8
JournalBioresource technology
Volume259
DOIs
Publication statusPublished - 2018 Jul

Fingerprint

Bentonite
Biocatalysts
chlorophenol
Biodegradation
Clay minerals
montmorillonite
biodegradation
Enzymes
enzyme
immobilization
Dioxygenases
Immobilized Enzymes
Cloning
Enzyme activity
Mixed Function Oxygenases
Purification
fulvic acid
Catalyst supports
Genes
enzyme activity

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Kwean, Oh Sung ; Cho, Su Yeon ; Yang, Jun Won ; Cho, Wooyoun ; Park, Sungyoon ; Lim, Yejee ; Shin, Min Chul ; Kim, Han Suk ; Park, Joonhong ; Kim, Han S. / 4-Chlorophenol biodegradation facilitator composed of recombinant multi-biocatalysts immobilized onto montmorillonite. In: Bioresource technology. 2018 ; Vol. 259. pp. 268-275.
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4-Chlorophenol biodegradation facilitator composed of recombinant multi-biocatalysts immobilized onto montmorillonite. / Kwean, Oh Sung; Cho, Su Yeon; Yang, Jun Won; Cho, Wooyoun; Park, Sungyoon; Lim, Yejee; Shin, Min Chul; Kim, Han Suk; Park, Joonhong; Kim, Han S.

In: Bioresource technology, Vol. 259, 07.2018, p. 268-275.

Research output: Contribution to journalArticle

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AU - Park, Sungyoon

AU - Lim, Yejee

AU - Shin, Min Chul

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