5-ALA induced fluorescent image analysis of actinic keratosis

Yong Jin Cho, Youngwoo Bae, Eung Ho Choi, Byungjo Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we quantitatively analyzed 5-ALA induced fluorescent images of actinic keratosis using digital fluorescent color and hyperspectral imaging modalities. UV-A was utilized to induce fluorescent images and actinic keratosis (AK) lesions were demarcated from surrounding the normal region with different methods. Eight subjects with AK lesion were participated in this study. In the hyperspectral imaging modality, spectral analysis method was utilized for hyperspectral cube image and AK lesions were demarcated from the normal region. Before image acquisition, we designated biopsy position for histopathology of AK lesion and surrounding normal region. Erythema index (E.I.) values on both regions were calculated from the spectral cube data. Image analysis of subjects resulted in two different groups: the first group with the higher fluorescence signal and E.I. on AK lesion than the normal region; the second group with lower fluorescence signal and without big difference in E.I. between two regions. In fluorescent color image analysis of facial AK, E.I. images were calculated on both normal and AK lesions and compared with the results of hyperspectral imaging modality. The results might indicate that the different intensity of fluorescence and E.I. among the subjects with AK might be interpreted as different phases of morphological and metabolic changes of AK lesions.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics VI
DOIs
Publication statusPublished - 2010 Dec 1
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics VI
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

Fingerprint

Actinic Keratosis
image analysis
lesions
Image analysis
Fluorescence
Erythema
Color image processing
Biopsy
Image acquisition
Spectrum analysis
fluorescence
Color
color
Hyperspectral imaging
spectrum analysis
acquisition

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Cho, Y. J., Bae, Y., Choi, E. H., & Jung, B. (2010). 5-ALA induced fluorescent image analysis of actinic keratosis. In Photonic Therapeutics and Diagnostics VI [75480J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7548). https://doi.org/10.1117/12.843050
Cho, Yong Jin ; Bae, Youngwoo ; Choi, Eung Ho ; Jung, Byungjo. / 5-ALA induced fluorescent image analysis of actinic keratosis. Photonic Therapeutics and Diagnostics VI. 2010. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{2bd87a2a6e8c4e0da0de8054bdaf9ef2,
title = "5-ALA induced fluorescent image analysis of actinic keratosis",
abstract = "In this study, we quantitatively analyzed 5-ALA induced fluorescent images of actinic keratosis using digital fluorescent color and hyperspectral imaging modalities. UV-A was utilized to induce fluorescent images and actinic keratosis (AK) lesions were demarcated from surrounding the normal region with different methods. Eight subjects with AK lesion were participated in this study. In the hyperspectral imaging modality, spectral analysis method was utilized for hyperspectral cube image and AK lesions were demarcated from the normal region. Before image acquisition, we designated biopsy position for histopathology of AK lesion and surrounding normal region. Erythema index (E.I.) values on both regions were calculated from the spectral cube data. Image analysis of subjects resulted in two different groups: the first group with the higher fluorescence signal and E.I. on AK lesion than the normal region; the second group with lower fluorescence signal and without big difference in E.I. between two regions. In fluorescent color image analysis of facial AK, E.I. images were calculated on both normal and AK lesions and compared with the results of hyperspectral imaging modality. The results might indicate that the different intensity of fluorescence and E.I. among the subjects with AK might be interpreted as different phases of morphological and metabolic changes of AK lesions.",
author = "Cho, {Yong Jin} and Youngwoo Bae and Choi, {Eung Ho} and Byungjo Jung",
year = "2010",
month = "12",
day = "1",
doi = "10.1117/12.843050",
language = "English",
isbn = "9780819479440",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Photonic Therapeutics and Diagnostics VI",

}

Cho, YJ, Bae, Y, Choi, EH & Jung, B 2010, 5-ALA induced fluorescent image analysis of actinic keratosis. in Photonic Therapeutics and Diagnostics VI., 75480J, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7548, Photonic Therapeutics and Diagnostics VI, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.843050

5-ALA induced fluorescent image analysis of actinic keratosis. / Cho, Yong Jin; Bae, Youngwoo; Choi, Eung Ho; Jung, Byungjo.

Photonic Therapeutics and Diagnostics VI. 2010. 75480J (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7548).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - 5-ALA induced fluorescent image analysis of actinic keratosis

AU - Cho, Yong Jin

AU - Bae, Youngwoo

AU - Choi, Eung Ho

AU - Jung, Byungjo

PY - 2010/12/1

Y1 - 2010/12/1

N2 - In this study, we quantitatively analyzed 5-ALA induced fluorescent images of actinic keratosis using digital fluorescent color and hyperspectral imaging modalities. UV-A was utilized to induce fluorescent images and actinic keratosis (AK) lesions were demarcated from surrounding the normal region with different methods. Eight subjects with AK lesion were participated in this study. In the hyperspectral imaging modality, spectral analysis method was utilized for hyperspectral cube image and AK lesions were demarcated from the normal region. Before image acquisition, we designated biopsy position for histopathology of AK lesion and surrounding normal region. Erythema index (E.I.) values on both regions were calculated from the spectral cube data. Image analysis of subjects resulted in two different groups: the first group with the higher fluorescence signal and E.I. on AK lesion than the normal region; the second group with lower fluorescence signal and without big difference in E.I. between two regions. In fluorescent color image analysis of facial AK, E.I. images were calculated on both normal and AK lesions and compared with the results of hyperspectral imaging modality. The results might indicate that the different intensity of fluorescence and E.I. among the subjects with AK might be interpreted as different phases of morphological and metabolic changes of AK lesions.

AB - In this study, we quantitatively analyzed 5-ALA induced fluorescent images of actinic keratosis using digital fluorescent color and hyperspectral imaging modalities. UV-A was utilized to induce fluorescent images and actinic keratosis (AK) lesions were demarcated from surrounding the normal region with different methods. Eight subjects with AK lesion were participated in this study. In the hyperspectral imaging modality, spectral analysis method was utilized for hyperspectral cube image and AK lesions were demarcated from the normal region. Before image acquisition, we designated biopsy position for histopathology of AK lesion and surrounding normal region. Erythema index (E.I.) values on both regions were calculated from the spectral cube data. Image analysis of subjects resulted in two different groups: the first group with the higher fluorescence signal and E.I. on AK lesion than the normal region; the second group with lower fluorescence signal and without big difference in E.I. between two regions. In fluorescent color image analysis of facial AK, E.I. images were calculated on both normal and AK lesions and compared with the results of hyperspectral imaging modality. The results might indicate that the different intensity of fluorescence and E.I. among the subjects with AK might be interpreted as different phases of morphological and metabolic changes of AK lesions.

UR - http://www.scopus.com/inward/record.url?scp=79751485901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79751485901&partnerID=8YFLogxK

U2 - 10.1117/12.843050

DO - 10.1117/12.843050

M3 - Conference contribution

AN - SCOPUS:79751485901

SN - 9780819479440

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Photonic Therapeutics and Diagnostics VI

ER -

Cho YJ, Bae Y, Choi EH, Jung B. 5-ALA induced fluorescent image analysis of actinic keratosis. In Photonic Therapeutics and Diagnostics VI. 2010. 75480J. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.843050