5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

Han Goo Kang, Seungjae Kim, Kwanghoon Lee, Shan Jin, Seo Hyeong Kim, Kangtaek Lee, Hyungjoon Jeon, Young Geon Song, Sang Won Lee, Jinwon Seo, Soomin Park, In Hong Choi

Research output: Contribution to journalArticle

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Abstract

The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca++ in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.

Original languageEnglish
Article number1602363
JournalSmall
Volume13
Issue number9
DOIs
Publication statusPublished - 2017 Mar 7

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Atopic Dermatitis
Allergens
Silver
Mast Cells
Nanoparticles
Hypersensitivity
Allergies
Cell Degranulation
Tryptases
Mites
Hydrogen peroxide
Superoxides
Immunoglobulin E
Hydrogen Peroxide
Reactive Oxygen Species
Skin
Chemical activation
Oxygen
Serum

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

Cite this

Kang, Han Goo ; Kim, Seungjae ; Lee, Kwanghoon ; Jin, Shan ; Kim, Seo Hyeong ; Lee, Kangtaek ; Jeon, Hyungjoon ; Song, Young Geon ; Lee, Sang Won ; Seo, Jinwon ; Park, Soomin ; Choi, In Hong. / 5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells. In: Small. 2017 ; Vol. 13, No. 9.
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abstract = "The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca++ in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.",
author = "Kang, {Han Goo} and Seungjae Kim and Kwanghoon Lee and Shan Jin and Kim, {Seo Hyeong} and Kangtaek Lee and Hyungjoon Jeon and Song, {Young Geon} and Lee, {Sang Won} and Jinwon Seo and Soomin Park and Choi, {In Hong}",
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Kang, HG, Kim, S, Lee, K, Jin, S, Kim, SH, Lee, K, Jeon, H, Song, YG, Lee, SW, Seo, J, Park, S & Choi, IH 2017, '5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells', Small, vol. 13, no. 9, 1602363. https://doi.org/10.1002/smll.201602363

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells. / Kang, Han Goo; Kim, Seungjae; Lee, Kwanghoon; Jin, Shan; Kim, Seo Hyeong; Lee, Kangtaek; Jeon, Hyungjoon; Song, Young Geon; Lee, Sang Won; Seo, Jinwon; Park, Soomin; Choi, In Hong.

In: Small, Vol. 13, No. 9, 1602363, 07.03.2017.

Research output: Contribution to journalArticle

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AU - Kang, Han Goo

AU - Kim, Seungjae

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AU - Jin, Shan

AU - Kim, Seo Hyeong

AU - Lee, Kangtaek

AU - Jeon, Hyungjoon

AU - Song, Young Geon

AU - Lee, Sang Won

AU - Seo, Jinwon

AU - Park, Soomin

AU - Choi, In Hong

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N2 - The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca++ in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.

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