5S rRNA-assisted DnaK refolding

Hyo Kyung Kim, Seong Il Choi, Baik L. Seong

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Although accumulating evidence has revealed that most proteins can fold without the assistance of molecular chaperones, little attention has been paid to other types of chaperoning macromolecules. A variety of proteins interact with diverse RNA molecules in vivo, suggesting a potential role of RNAs for folding of their interacting proteins. Here we show that the in vitro refolding of a representative molecular chaperone, DnaK, an Escherichia coli homolog of Hsp70, could be assisted by its interacting 5S rRNA. The folding enhancement occurred in RNA concentration and its size dependent manner whereas neither the RNA with the reverse sequence of 5S rRNA nor the RNase pretreated 5S rRNA stimulated the folding in vitro. Based on our results, we propose that 5S rRNA could exert the chaperoning activity on DnaK during the folding process. The results suggest an interesting possibility that the folding of RNA-interacting proteins could be assisted by their cognate RNA ligands.

Original languageEnglish
Pages (from-to)1177-1181
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number2
DOIs
Publication statusPublished - 2010 Jan 8

Bibliographical note

Funding Information:
This work was supported by a grant from the Ministry of Knowledge and Economy of Korean Government (Grant No. 10031969 ) and the Translational Research Center for Protein Function Control from National Research Foundation of Korea .

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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