A 12,800-well single-cell-derived sphere assay chip utilizing highly-parallel microfluidic structures for cancer heterogeneity studies

Yu Heng Cheng, Yu Chih Chen, Riley Brien, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recent research indicates that cancer stem-like cells (CSCs) are the key subpopulation for tumor relapse and metastasis. Due to cancer plasticity in surface antigen and enzymatic activity markers, functional tumorsphere assays are promising alternatives for CSC identification. To reliably quantify rare CSCs (1-5%), thousands of single-cell suspension cultures are needed. While microfluidics is a powerful tool in handling single cells, previous works provided a limited throughput and lack in automatic data analysis required for high-throughput assays. In this work, we present a highthroughput single-cell-derived tumor sphere assay chip, facilitating the tracking of up to ∼10,000 cells in each chip with ∼76.5% capture rate. The presented cell capture scheme guarantees sampling of a representative population from the bulk cells. Combined with F108 coating on PDMS, a suspension culture environment was created for high-throughput tumorsphere assays. To analyze thousands of single-cells, a highly adaptable analysis program was developed for cell/sphere counting and size measurement for automation in CSC heterogeneity studies.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages238-239
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Cheng, Y. H., Chen, Y. C., Brien, R., & Yoon, E. (2016). A 12,800-well single-cell-derived sphere assay chip utilizing highly-parallel microfluidic structures for cancer heterogeneity studies. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 238-239). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.