A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels

Kiwon Song, Sang Hyeon Im, Yeo Jun Yoon, Hui Min Kim, Hae June Lee, Gwan Soo Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Previously, we showed that exposure of human normal and cancer cells to a 6 mT, 60 Hz gradient electromagnetic field (EMF) induced genotoxicity. Here, we investigated the cellular effects of a uniform EMF. Single or repetitive exposure to a 6 mT, 60 Hz uniform EMF neither induced DNA damage nor affected cell viability in HeLa and primary IMR-90 fibroblasts. However, continuous exposure of these cells to an EMF promoted cell proliferation. Cell viability increased 24.4% for HeLa and 15.2% for IMR-90 cells after a total 168 h exposure by subculture. This increase in cell proliferation was directly correlated with EMF strength and exposure time. When further incubated without EMF, cell proliferation slowed down to that of unexposed cells, suggesting that the proliferative effect is reversible. The expression of cell cycle markers increased in cells continuously exposed to an EMF as expected, but the distribution of cells in each stage of the cell cycle did not change. Notably, intracellular reactive oxygen species levels decreased and phosphorylation of Akt and Erk1/2 increased in cells exposed to an EMF, suggesting that reduced levels of intracellular reactive oxygen species play a role in increased proliferation. These results demonstrate that EMF uniformity at an extremely low frequency (ELF) is an important factor in the cellular effects of ELF-EMF.

Original languageEnglish
Article numbere0199753
JournalPloS one
Volume13
Issue number7
DOIs
Publication statusPublished - 2018 Jul

Fingerprint

Electromagnetic Fields
Cell proliferation
Electromagnetic fields
reactive oxygen species
Reactive Oxygen Species
cell proliferation
Cell Proliferation
Cells
cells
cell viability
cell cycle
Cell Survival
Cell Cycle
electromagnetic field
Phosphorylation
genotoxicity
Fibroblasts
DNA damage
DNA Damage
exposure duration

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Song, Kiwon ; Im, Sang Hyeon ; Yoon, Yeo Jun ; Kim, Hui Min ; Lee, Hae June ; Park, Gwan Soo. / A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels. In: PloS one. 2018 ; Vol. 13, No. 7.
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A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels. / Song, Kiwon; Im, Sang Hyeon; Yoon, Yeo Jun; Kim, Hui Min; Lee, Hae June; Park, Gwan Soo.

In: PloS one, Vol. 13, No. 7, e0199753, 07.2018.

Research output: Contribution to journalArticle

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