A beneficial effect of simvastatin on DNA damage in 242T allele of the NADPH oxidase p22phox in hypercholesterolemic patients

Min Jeong Shin, Young Cho Eun, Yangsoo Jang, Ho Lee Jong, Won Heum Shim, Seung Yun Cho, Se Joong Rim, Seok Min Kang, Jong Won Ha, Young Guk Ko, Sung Soon Kim, Young Park Hyun, Namsik Chung

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Abstract

Background: The effect of simvastatin on DNA damage in hypercholesterolemic patients was investigated, and the relationship between the C242T polymorphism of the NADPH oxidase p22phox gene and the antioxidant effects of simvastatin was examined. Methods: Simvastatin (20-40 mg /day) was administered for 8 weeks in 72 hypercholesterolemic patients. DNA damage in lymphocytes was quantified using single-cell gel electrophoresis (COMET assay) by measuring tail DNA (%), tail length (μm) and tail moment (tail length × % tail DNA / 100). Results: Simvastatin significantly reduced DNA damage as expressed by tail DNA (%, p < 0.001), tail length (μm, p < 0.001) and tail moment on the DNA in lymphocytes (p < 0.001) after 8 weeks. The frequencies of the C242T genotypes for CC, TC, and TT were 75.0%, 23.6% and 1.4% in the subjects. In the presence of the 242T allele, there were higher levels of baseline DNA damage and also a greater improvement in the DNA damage after 8 week simvastatin treatment compared with the CC homozygotes. Conclusion: Simvastatin significantly reduced DNA damage of hypercholesterolemic patients. This study showed that simvastatin has a beneficial effect on the improvement of DNA damage in patients with the 242T allele of NADPH oxidase p22phox gene.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalClinica Chimica Acta
Volume360
Issue number1-2
DOIs
Publication statusPublished - 2005 Oct 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Shin, M. J., Eun, Y. C., Jang, Y., Jong, H. L., Shim, W. H., Cho, S. Y., Rim, S. J., Kang, S. M., Ha, J. W., Ko, Y. G., Kim, S. S., Hyun, Y. P., & Chung, N. (2005). A beneficial effect of simvastatin on DNA damage in 242T allele of the NADPH oxidase p22phox in hypercholesterolemic patients. Clinica Chimica Acta, 360(1-2), 46-51. https://doi.org/10.1016/j.cccn.2005.04.001