A better yield of islet cell mass from living pancreatic donors compared with cadaveric donors

Hye Seung Jung, Seong Ho Choi, Sung Joo Kim, Kyu Taek Lee, Jong Kyun Lee, Kee Taek Jang, Byung Wan Lee, Jae Hwan Jee, Seung Hoon Oh, You Ran Ahn, Moon Kyu Lee, Kwang Won Kim

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21 Citations (Scopus)

Abstract

Studies in rats have shown that brain death decreases β-cell function and causes islet cell death during islet isolation and transplantation. Because a direct comparison of human islet cells between living and cadaveric donors has not been reported to date, we studied the effects of brain death on islet cell yield. A total of 36 pancreas specimens from 20 living donors and 16 cadaveric donors were used for analysis. Islets were isolated with a Ricordi chamber, and counted as equivalent islet numbers (EIN). Living donors were predominantly female, and cadaveric donors were mainly male. Although the cold ischemic time, pancreas distensibility and digestion time were not different, islet yield was observed to be higher in living donors compared with cadaveric donors (5800 ± 3500 vs. 1900 ± 2000EIN/g pancreas). Islet isolation success rates (when defined as more than 2000EIN/g) were 94.1% and 42.9%, respectively. Post-Ficoll islet recovery rates and purity were also better in living donors. However, islet viability and in vitro function of isolated islets showed no significant differences between the groups. These results suggested that brain death negatively affected the processes of islet isolation from the pancreas.

Original languageEnglish
Pages (from-to)738-743
Number of pages6
JournalClinical Transplantation
Volume21
Issue number6
DOIs
Publication statusPublished - 2007 Nov 1

All Science Journal Classification (ASJC) codes

  • Transplantation

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    Jung, H. S., Choi, S. H., Kim, S. J., Lee, K. T., Lee, J. K., Jang, K. T., Lee, B. W., Jee, J. H., Oh, S. H., Ahn, Y. R., Lee, M. K., & Kim, K. W. (2007). A better yield of islet cell mass from living pancreatic donors compared with cadaveric donors. Clinical Transplantation, 21(6), 738-743. https://doi.org/10.1111/j.1399-0012.2007.00731.x