A bone replaceable artificial bone substitute: Osteoinduction by combining with bone inducing agent

Hwal Suh, Dong Wook Han, Jongchul Park, Dong Hee Lee, Woo Suk Lee, Chang Dong Han

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bone inducing agent (BIA) isolated from Saos-2 human osteosarcoma cells was added to an artificial bone substitute composed of 980°C-heated carbonate apatite (CAp) and Type I atelocollagen (AtCol) extracted from bovine tail skins (88/12 in wt/wt%), and a CAp-AtCol-BIA substitute was prepared as an osteoinductive bone substitute. Rat calvaria osteoblasts treated by the isolated BIA demonstrated significantly increased alkaline phosphatase (ALP) activity after 3 days (p < 0.05). In vitro cell attachment and proliferation and ALP activity were investigated for the bone substitute combined with BIA. Osteoblasts cultured onto the surface of the CAp-AtCol-BIA substitute demonstrated remarkable morphological changes such as radial spreading, flattening, and projecting filopodia after 5 days. In comparison with the substitute without BIA, osteoblasts grown in the BIA-combined CAp-AtCol substitute expressed significantly increased proliferation and ALP activity, respectively (p < 0.05). Both the substitutes combined with and without BIA were implanted into artificial defects created in rabbit radii. After 4 weeks, the CAp-AtCol-BIA substitute implanted lesion was completely replaced by regenerated host bone in radiological observation whereas the substitute without BIA was partially resorbed. No histologic abnormalities appeared in the substitute either with or without BIA.

Original languageEnglish
Pages (from-to)459-466
Number of pages8
JournalArtificial Organs
Volume25
Issue number6
DOIs
Publication statusPublished - 2001 Sep 29

Fingerprint

Bone Substitutes
Bone
Bone and Bones
Apatite
Osteoblasts
Carbonates
Alkaline Phosphatase
Phosphatases
Pseudopodia
Osteosarcoma
Skull
Tail
Cell Proliferation
Observation
atelocollagen
carboapatite
Rats
Skin
Rabbits

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Suh, Hwal ; Han, Dong Wook ; Park, Jongchul ; Lee, Dong Hee ; Lee, Woo Suk ; Han, Chang Dong. / A bone replaceable artificial bone substitute : Osteoinduction by combining with bone inducing agent. In: Artificial Organs. 2001 ; Vol. 25, No. 6. pp. 459-466.
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A bone replaceable artificial bone substitute : Osteoinduction by combining with bone inducing agent. / Suh, Hwal; Han, Dong Wook; Park, Jongchul; Lee, Dong Hee; Lee, Woo Suk; Han, Chang Dong.

In: Artificial Organs, Vol. 25, No. 6, 29.09.2001, p. 459-466.

Research output: Contribution to journalArticle

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