A calorimetric biosensor and its application for detecting a cancer cell with optical imaging

Se Chul Park, Eun Jin Cho, Se Young Moon, Seung Il Yoon, Yong-Jun Kim, Donghyun Kim, Jinsuck Suh

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

This paper reports a biochemical sensor for a label-free detection of cancer cell. A microcalorimeter has monitored the signal created from generated heat due to biochemical reaction between cancer cell and antibody in real time. HER2/neu receptor which is a remarkable biomarker of several cancers, especially breast cancer, was detected by using antibody, in this case HERCEPTIN®. In this study, the microcalorimeter was fabricated by using MEMS (Micro Electro Mechanical Systems)-technology. For higher sensitivity of microcalorimeter, it is important to reduce a parasitic heat transfer from a thermopile to a silicon substrate which has higher thermal conductivity (k = 148 W/m·K). Hot junctions of the proposed microcalorimeter are released from a silicon substrate by bulk micromachining to reduce loss of generated heat by reactions between biomolecules. NIH3T6.7 cell line with overexpressed HER2/neu receptor and FITC conjugated HERCEPTIN® were used. Each volume of biomolecules was 10 μl. HER2/neu receptor was detected by measuring output voltage change of the microcalorimeter about 7.92V/mole. For confirming a reliability of data, the measured data was compared with fluorescent data.

Original languageEnglish
Pages (from-to)637-640
Number of pages4
JournalIFMBE Proceedings
Volume14
Issue number1
Publication statusPublished - 2007 Jan 1
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Fingerprint

ErbB-2 Receptor
Biosensors
Cells
Biomolecules
Silicon
Imaging techniques
Antibodies
Thermopiles
Fluorescein-5-isothiocyanate
Micromachining
Biomarkers
Substrates
Tumor Biomarkers
Labels
Thermal conductivity
Heat transfer
Sensors
Electric potential
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Park, Se Chul ; Cho, Eun Jin ; Moon, Se Young ; Yoon, Seung Il ; Kim, Yong-Jun ; Kim, Donghyun ; Suh, Jinsuck. / A calorimetric biosensor and its application for detecting a cancer cell with optical imaging. In: IFMBE Proceedings. 2007 ; Vol. 14, No. 1. pp. 637-640.
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A calorimetric biosensor and its application for detecting a cancer cell with optical imaging. / Park, Se Chul; Cho, Eun Jin; Moon, Se Young; Yoon, Seung Il; Kim, Yong-Jun; Kim, Donghyun; Suh, Jinsuck.

In: IFMBE Proceedings, Vol. 14, No. 1, 01.01.2007, p. 637-640.

Research output: Contribution to journalConference article

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