A carbohydrate antigen of Clonorchis sinensis recognized by a species-specific monoclonal antibody.

T. S. Yong, J. S. Lee, S. N. Cho, J. H. Seo, H. Park

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Abstract

The enzyme-linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody (MAb), CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. sinensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the MAb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.

Original languageEnglish
Pages (from-to)279-281
Number of pages3
JournalThe Korean journal of parasitology
Volume34
Issue number4
DOIs
Publication statusPublished - 1996 Dec

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All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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