A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum

Jeseung Oh, Gu Yoo, Young Wook Chang, Hyung Joon Kim, Joachim Jose, Eosu Kim, Jae Chul Pyun, Kyung Hwa Yoo

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-β (Aβ) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. Aβ at the level of 1. pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma Aβ using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalBiosensors and Bioelectronics
Volume50
DOIs
Publication statusPublished - 2013 Dec 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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