A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum

Jeseung Oh, Gu Yoo, Young Wook Chang, Hyung Joon Kim, Joachim Jose, Eosu Kim, Jae Chul Pyun, Kyung Hwa Yoo

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-β (Aβ) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. Aβ at the level of 1. pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma Aβ using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalBiosensors and Bioelectronics
Volume50
DOIs
Publication statusPublished - 2013 Dec 15

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MESFET devices
Carbon nanotube field effect transistors
Semiconductors
Carbon Nanotubes
Biosensing Techniques
Amyloid
Biosensors
Antibodies
Metals
Immobilized Antibodies
Escherichia coli
Carbon nanotubes
Serum
Membranes
Gold
Carrier Proteins
Early Diagnosis
Alzheimer Disease
Proteins
Staphylococcal Protein A

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Oh, Jeseung ; Yoo, Gu ; Chang, Young Wook ; Kim, Hyung Joon ; Jose, Joachim ; Kim, Eosu ; Pyun, Jae Chul ; Yoo, Kyung Hwa. / A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum. In: Biosensors and Bioelectronics. 2013 ; Vol. 50. pp. 345-350.
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A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum. / Oh, Jeseung; Yoo, Gu; Chang, Young Wook; Kim, Hyung Joon; Jose, Joachim; Kim, Eosu; Pyun, Jae Chul; Yoo, Kyung Hwa.

In: Biosensors and Bioelectronics, Vol. 50, 15.12.2013, p. 345-350.

Research output: Contribution to journalArticle

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