A case of scedosporium apiospermum keratitis confirmed by a molecular genetic method

Seoyoung Yoon, Sinyoung Kim, Kyung A. Lee, Heejung Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A 54-yr-old male, who was treated by chemotherapy for gastric cancer 15 months ago, presented to Yongdong Severance Hospital, Seoul, with complaints of pain in his right eye caused by a foreign body from the ground in the previous week. He had been treated with topical and oral antibacterial in addition to antifungal agents, but did not show significant clinical improvement. After a positive corneal culture with mold, topical amphotericin B was added to the initial regimen. The mold was identified as Scedosporium apiospermum by macroscopic and microscopic morphologies and the nucleotide sequences of a fungal PCR product showing 99% homology with those of S. apiospermum (EF151349). He recovered with good results at 25 days after corneal epithelial debridement. The early diagnosis of S. apiospermum keratitis is very important for proper treatment. It is recommended that molecular diagnostic methods such as fungal PCR and sequencing be done with conventional cultures whenever a fungal infection is suspected.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalKorean Journal of Laboratory Medicine
Volume28
Issue number4
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Scedosporium
Chemotherapy
Keratitis
Antifungal Agents
Amphotericin B
Molecular Biology
Nucleotides
Fungi
Polymerase Chain Reaction
Molecular Pathology
Mycoses
Debridement
Foreign Bodies
Stomach Neoplasms
Early Diagnosis
Drug Therapy
Pain
Therapeutics

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Yoon, Seoyoung ; Kim, Sinyoung ; Lee, Kyung A. ; Kim, Heejung. / A case of scedosporium apiospermum keratitis confirmed by a molecular genetic method. In: Korean Journal of Laboratory Medicine. 2008 ; Vol. 28, No. 4. pp. 307-311.
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A case of scedosporium apiospermum keratitis confirmed by a molecular genetic method. / Yoon, Seoyoung; Kim, Sinyoung; Lee, Kyung A.; Kim, Heejung.

In: Korean Journal of Laboratory Medicine, Vol. 28, No. 4, 01.12.2008, p. 307-311.

Research output: Contribution to journalArticle

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AB - A 54-yr-old male, who was treated by chemotherapy for gastric cancer 15 months ago, presented to Yongdong Severance Hospital, Seoul, with complaints of pain in his right eye caused by a foreign body from the ground in the previous week. He had been treated with topical and oral antibacterial in addition to antifungal agents, but did not show significant clinical improvement. After a positive corneal culture with mold, topical amphotericin B was added to the initial regimen. The mold was identified as Scedosporium apiospermum by macroscopic and microscopic morphologies and the nucleotide sequences of a fungal PCR product showing 99% homology with those of S. apiospermum (EF151349). He recovered with good results at 25 days after corneal epithelial debridement. The early diagnosis of S. apiospermum keratitis is very important for proper treatment. It is recommended that molecular diagnostic methods such as fungal PCR and sequencing be done with conventional cultures whenever a fungal infection is suspected.

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