A chloroplast-targeted pentatricopeptide repeat protein PPR287 is crucial for chloroplast function and Arabidopsis development

Kwanuk Lee, Su Jung Park, Ji Hoon Han, Young Jeon, Hyunsook Pai, Hunseung Kang

Research output: Contribution to journalArticle

Abstract

Background: Even though the roles of pentatricopeptide repeat (PPR) proteins are essential in plant organelles, the function of many chloroplast-targeted PPR proteins remains unknown. Here, we characterized the function of a chloroplast-localized PPR protein (At3g59040), which is classified as the 287th PPR protein among the 450 PPR proteins in Arabidopsis (http://ppr.plantenergy.uwa.edu.au). Results: The homozygous ppr287 mutant with the T-DNA inserted into the last exon displayed pale-green and yellowish phenotypes. The microRNA-mediated knockdown mutants were generated to further confirm the developmental defect phenotypes of ppr287 mutants. All mutants had yellowish leaves, shorter roots and height, and less seed yield, indicating that PPR287 is crucial for normal Arabidopsis growth and development. The photosynthetic activity and chlorophyll content of ppr287 mutants were markedly reduced, and the chloroplast structures of the mutants were abnormal. The levels of chloroplast rRNAs were decreased in ppr287 mutants. Conclusions: These results suggest that PPR287 plays an essential role in chloroplast biogenesis and function, which is crucial for the normal growth and development of Arabidopsis.

Original languageEnglish
Article number244
JournalBMC Plant Biology
Volume19
Issue number1
DOIs
Publication statusPublished - 2019 Jun 7

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chloroplasts
Arabidopsis
mutants
proteins
growth and development
phenotype
microRNA
seed yield
exons
organelles
ribosomal RNA
chlorophyll
DNA
leaves

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Lee, Kwanuk ; Park, Su Jung ; Han, Ji Hoon ; Jeon, Young ; Pai, Hyunsook ; Kang, Hunseung. / A chloroplast-targeted pentatricopeptide repeat protein PPR287 is crucial for chloroplast function and Arabidopsis development. In: BMC Plant Biology. 2019 ; Vol. 19, No. 1.
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abstract = "Background: Even though the roles of pentatricopeptide repeat (PPR) proteins are essential in plant organelles, the function of many chloroplast-targeted PPR proteins remains unknown. Here, we characterized the function of a chloroplast-localized PPR protein (At3g59040), which is classified as the 287th PPR protein among the 450 PPR proteins in Arabidopsis (http://ppr.plantenergy.uwa.edu.au). Results: The homozygous ppr287 mutant with the T-DNA inserted into the last exon displayed pale-green and yellowish phenotypes. The microRNA-mediated knockdown mutants were generated to further confirm the developmental defect phenotypes of ppr287 mutants. All mutants had yellowish leaves, shorter roots and height, and less seed yield, indicating that PPR287 is crucial for normal Arabidopsis growth and development. The photosynthetic activity and chlorophyll content of ppr287 mutants were markedly reduced, and the chloroplast structures of the mutants were abnormal. The levels of chloroplast rRNAs were decreased in ppr287 mutants. Conclusions: These results suggest that PPR287 plays an essential role in chloroplast biogenesis and function, which is crucial for the normal growth and development of Arabidopsis.",
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A chloroplast-targeted pentatricopeptide repeat protein PPR287 is crucial for chloroplast function and Arabidopsis development. / Lee, Kwanuk; Park, Su Jung; Han, Ji Hoon; Jeon, Young; Pai, Hyunsook; Kang, Hunseung.

In: BMC Plant Biology, Vol. 19, No. 1, 244, 07.06.2019.

Research output: Contribution to journalArticle

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AU - Kang, Hunseung

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