Abstract
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
| Original language | English |
|---|---|
| Pages (from-to) | 3434-3442 |
| Number of pages | 9 |
| Journal | Biomedical Optics Express |
| Volume | 5 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2014 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics
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A compact Airy beam light sheet microscope with a tilted cylindrical lens. / Yang, Zhengyi; Prokopas, Martynas; Nylk, Jonathan; Coll-Lladó, Clara; Gunn-Moore, Frank J.; Ferrier, David E.K.; Vettenburg, Tom; Dholakia, Kishan.
In: Biomedical Optics Express, Vol. 5, No. 10, 01.10.2014, p. 3434-3442.Research output: Contribution to journal › Article
TY - JOUR
T1 - A compact Airy beam light sheet microscope with a tilted cylindrical lens
AU - Yang, Zhengyi
AU - Prokopas, Martynas
AU - Nylk, Jonathan
AU - Coll-Lladó, Clara
AU - Gunn-Moore, Frank J.
AU - Ferrier, David E.K.
AU - Vettenburg, Tom
AU - Dholakia, Kishan
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
AB - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
UR - http://www.scopus.com/inward/record.url?scp=84942374722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942374722&partnerID=8YFLogxK
U2 - 10.1364/BOE.5.003434
DO - 10.1364/BOE.5.003434
M3 - Article
AN - SCOPUS:84942374722
VL - 5
SP - 3434
EP - 3442
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 10
ER -