A compact Airy beam light sheet microscope with a tilted cylindrical lens

Zhengyi Yang, Martynas Prokopas, Jonathan Nylk, Clara Coll-Lladó, Frank J. Gunn-Moore, David E.K. Ferrier, Tom Vettenburg, Kishan Dholakia

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

Original languageEnglish
Pages (from-to)3434-3442
Number of pages9
JournalBiomedical Optics Express
Volume5
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

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light beams
Lenses
microscopes
lenses
Light
larvae
propagation
Costs and Cost Analysis
footprints
light modulators
programming
Zebrafish
field of view
Lighting
Microspheres
Photons
Sepharose
Larva
illumination
costs

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Yang, Z., Prokopas, M., Nylk, J., Coll-Lladó, C., Gunn-Moore, F. J., Ferrier, D. E. K., ... Dholakia, K. (2014). A compact Airy beam light sheet microscope with a tilted cylindrical lens. Biomedical Optics Express, 5(10), 3434-3442. https://doi.org/10.1364/BOE.5.003434
Yang, Zhengyi ; Prokopas, Martynas ; Nylk, Jonathan ; Coll-Lladó, Clara ; Gunn-Moore, Frank J. ; Ferrier, David E.K. ; Vettenburg, Tom ; Dholakia, Kishan. / A compact Airy beam light sheet microscope with a tilted cylindrical lens. In: Biomedical Optics Express. 2014 ; Vol. 5, No. 10. pp. 3434-3442.
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Yang, Z, Prokopas, M, Nylk, J, Coll-Lladó, C, Gunn-Moore, FJ, Ferrier, DEK, Vettenburg, T & Dholakia, K 2014, 'A compact Airy beam light sheet microscope with a tilted cylindrical lens', Biomedical Optics Express, vol. 5, no. 10, pp. 3434-3442. https://doi.org/10.1364/BOE.5.003434

A compact Airy beam light sheet microscope with a tilted cylindrical lens. / Yang, Zhengyi; Prokopas, Martynas; Nylk, Jonathan; Coll-Lladó, Clara; Gunn-Moore, Frank J.; Ferrier, David E.K.; Vettenburg, Tom; Dholakia, Kishan.

In: Biomedical Optics Express, Vol. 5, No. 10, 01.10.2014, p. 3434-3442.

Research output: Contribution to journalArticle

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Yang Z, Prokopas M, Nylk J, Coll-Lladó C, Gunn-Moore FJ, Ferrier DEK et al. A compact Airy beam light sheet microscope with a tilted cylindrical lens. Biomedical Optics Express. 2014 Oct 1;5(10):3434-3442. https://doi.org/10.1364/BOE.5.003434