A compact Airy beam light sheet microscope with a tilted cylindrical lens

Zhengyi Yang, Martynas Prokopas, Jonathan Nylk, Clara Coll-Lladó, Frank J. Gunn-Moore, David E.K. Ferrier, Tom Vettenburg, Kishan Dholakia

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

Original languageEnglish
Pages (from-to)3434-3442
Number of pages9
JournalBiomedical Optics Express
Volume5
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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    Yang, Z., Prokopas, M., Nylk, J., Coll-Lladó, C., Gunn-Moore, F. J., Ferrier, D. E. K., Vettenburg, T., & Dholakia, K. (2014). A compact Airy beam light sheet microscope with a tilted cylindrical lens. Biomedical Optics Express, 5(10), 3434-3442. https://doi.org/10.1364/BOE.5.003434