Abstract
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
Original language | English |
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Pages (from-to) | 3434-3442 |
Number of pages | 9 |
Journal | Biomedical Optics Express |
Volume | 5 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2014 Oct 1 |
Bibliographical note
Publisher Copyright:© 2014 Optical Society of America.
All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics