A comparison of Ypet and firefly luciferase as reporter proteins for high-throughput screening

Sei Mee Yoon, Wan Namkung, Jinu Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

When Ypet was used as a reporter protein for highthroughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.

Original languageEnglish
Pages (from-to)2328-2330
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number11
DOIs
Publication statusPublished - 2013 Dec 6

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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