A correlated force-optical study on the self-assembly behavior of annexin V on model membranes

Effect of dye conjugation

Jason J. Han, Seung Han Park, Doo Wan Boo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have examined the self-assembled membrane-bound aggregates of two annexin V (A5) dye conjugates and compared them to those from native A5. Native A5 and FITC-labeled A5 (A5-FITC) both formed discrete well-defined crystalline monolayer domains of p6 symmetry. However, A5-FITC also showed additional domains with a corrugated appearance not observed in native A5. In contrast, Cy3-labeled A5 (A5-Cy3) showed a mixture of crystalline monolayer and irregular multilayered domains, with the ratio of the two types varying significantly from sample to sample, and also required a much longer incubation time than native A5 and A5-FITC. When A5-FITC and A5-Cy3 were co-incubated on the same bilayer, welldefined crystalline monolayer domains containing both A5-FITC and A5-Cy3 were consistently observed at a much shorter incubation time than that of pure A5-Cy3 alone, indicating that A5-FITC facilitates the inclusion of A5-Cy3. These results suggest that dye labels can affect A5 2D self-assembly and crystal formation on membrane surfaces.

Original languageEnglish
Pages (from-to)12175-12178
Number of pages4
JournalJournal of Physical Chemistry B
Volume112
Issue number39
DOIs
Publication statusPublished - 2008 Oct 2

Fingerprint

Fluorescein-5-isothiocyanate
Annexin A5
conjugation
Self assembly
self assembly
Monolayers
Coloring Agents
Dyes
dyes
Crystalline materials
membranes
Membranes
Crystal symmetry
Labels
Crystals
inclusions
symmetry
crystals

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

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abstract = "We have examined the self-assembled membrane-bound aggregates of two annexin V (A5) dye conjugates and compared them to those from native A5. Native A5 and FITC-labeled A5 (A5-FITC) both formed discrete well-defined crystalline monolayer domains of p6 symmetry. However, A5-FITC also showed additional domains with a corrugated appearance not observed in native A5. In contrast, Cy3-labeled A5 (A5-Cy3) showed a mixture of crystalline monolayer and irregular multilayered domains, with the ratio of the two types varying significantly from sample to sample, and also required a much longer incubation time than native A5 and A5-FITC. When A5-FITC and A5-Cy3 were co-incubated on the same bilayer, welldefined crystalline monolayer domains containing both A5-FITC and A5-Cy3 were consistently observed at a much shorter incubation time than that of pure A5-Cy3 alone, indicating that A5-FITC facilitates the inclusion of A5-Cy3. These results suggest that dye labels can affect A5 2D self-assembly and crystal formation on membrane surfaces.",
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A correlated force-optical study on the self-assembly behavior of annexin V on model membranes : Effect of dye conjugation. / Han, Jason J.; Park, Seung Han; Boo, Doo Wan.

In: Journal of Physical Chemistry B, Vol. 112, No. 39, 02.10.2008, p. 12175-12178.

Research output: Contribution to journalArticle

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