Photothermal sensors represent a novel type of probe having potential in the field of clinical diagnostics particularly due to the lack of a tedious sample pre-treatment, a sophisticated equipment and a skilled manpower. Herein, we describe the fabrication of a microfluidic nickel-resistive temperature detector (micro-Ni-RTD) for the selective monitoring of diabetes using a boronate-based sialic acid (SA) receptor. This work represents an improvement made to our previous photothermal sensor designs by reducing the cost by about 100-fold and significantly improving the sensitivity of the device, enabling the detection of sialic acid contents as low as 0.06 μmol/mL, a 4-fold improvement compared to the sensor described our previous study. The average temperature difference between healthy subjects and diabetes patients was found to be 0.181 °C dL/g with micro-Ni-RTD in comparison with 0.043 °C dL/g with our previous platinum-based resistive temperature detector (Pt-RTD) reported previously The enhancement in the discriminatory ability of micro-Ni-RTD for the diabetic clinical samples compared with healthy control can be attributed to the modified surface modification strategy and the incorporation of the microfluidic channels. Thus, micro-Ni-RTD represents a convenient, rapid, reliable, and low-cost biosensor for the clinical monitoring of diabetes patients.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2020R1A5A1018052 , 2018R1C1B6002499 , and 2018R1A2A2A15019814 ), and Korea Environment Industry & Technology Institute(KEITI) through Aquatic Ecosystem Conversion Research Program, funded by Korea Ministry of Environment(MOE) ( 2020003030007 ).
© 2020 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry