A cost-effective and sensitive photothermal biosensor for the diagnosis of diabetes based on quantifying the sialic acid content on erythrocytes

Kirok Kwon, Sudesna Chakravarty, Kyung A. Hyun, Nam Ho Bae, Jaewoo Song, Seok Jae Lee, Hyo Il Jung

Research output: Contribution to journalArticlepeer-review

Abstract

Photothermal sensors represent a novel type of probe having potential in the field of clinical diagnostics particularly due to the lack of a tedious sample pre-treatment, a sophisticated equipment and a skilled manpower. Herein, we describe the fabrication of a microfluidic nickel-resistive temperature detector (micro-Ni-RTD) for the selective monitoring of diabetes using a boronate-based sialic acid (SA) receptor. This work represents an improvement made to our previous photothermal sensor designs by reducing the cost by about 100-fold and significantly improving the sensitivity of the device, enabling the detection of sialic acid contents as low as 0.06 μmol/mL, a 4-fold improvement compared to the sensor described our previous study. The average temperature difference between healthy subjects and diabetes patients was found to be 0.181 °C dL/g with micro-Ni-RTD in comparison with 0.043 °C dL/g with our previous platinum-based resistive temperature detector (Pt-RTD) reported previously The enhancement in the discriminatory ability of micro-Ni-RTD for the diabetic clinical samples compared with healthy control can be attributed to the modified surface modification strategy and the incorporation of the microfluidic channels. Thus, micro-Ni-RTD represents a convenient, rapid, reliable, and low-cost biosensor for the clinical monitoring of diabetes patients.

Original languageEnglish
Article number129259
JournalSensors and Actuators, B: Chemical
Volume329
DOIs
Publication statusPublished - 2021 Feb 15

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2020R1A5A1018052 , 2018R1C1B6002499 , and 2018R1A2A2A15019814 ), and Korea Environment Industry & Technology Institute(KEITI) through Aquatic Ecosystem Conversion Research Program, funded by Korea Ministry of Environment(MOE) ( 2020003030007 ).

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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