A detection kit for Aeromonas hydrophila using antibody sensitized latex

S. Enjoo, S. Lee, Kyungwon Lee, Y. Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aeromonas hydrophila is a pathogen to fish as well as human. It is a food-borne disease, and causes severe mortality in fish, and sometimes severe septicemia in human. In this study, a rapid detection method using latex agglutination has been developed for A. hydrophila. Polyclonal antibodies were raised against membrane and whole cells of three isolates from rainbow trout. Among these, latex particles coated with antibodies raised against whole cells of isolate No. 2 showed the best sensitivity. With latex particles coated with this antibody, we could detect 5x104 CFU of A. hydrophila in 5 min. The cross-reactivity with bacteria constituting the normal intestinal microflora and other pathogens for rainbow trout was insignificant. This latex agglutination assay method produced positive reaction with all clinical isolates of A. hydrophila which were identified by species-specific PCR for 16S rRNA in A. hydrophila.

Original languageEnglish
Pages (from-to)595-598
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume10
Issue number5
Publication statusPublished - 2000 Jan 1

Fingerprint

Aeromonas hydrophila
Latex
Antibodies
Oncorhynchus mykiss
Agglutination
Microspheres
Fishes
Foodborne Diseases
Sepsis
Cell Membrane
Bacteria
Polymerase Chain Reaction
Mortality

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Aeromonas hydrophila is a pathogen to fish as well as human. It is a food-borne disease, and causes severe mortality in fish, and sometimes severe septicemia in human. In this study, a rapid detection method using latex agglutination has been developed for A. hydrophila. Polyclonal antibodies were raised against membrane and whole cells of three isolates from rainbow trout. Among these, latex particles coated with antibodies raised against whole cells of isolate No. 2 showed the best sensitivity. With latex particles coated with this antibody, we could detect 5x104 CFU of A. hydrophila in 5 min. The cross-reactivity with bacteria constituting the normal intestinal microflora and other pathogens for rainbow trout was insignificant. This latex agglutination assay method produced positive reaction with all clinical isolates of A. hydrophila which were identified by species-specific PCR for 16S rRNA in A. hydrophila.",
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A detection kit for Aeromonas hydrophila using antibody sensitized latex. / Enjoo, S.; Lee, S.; Lee, Kyungwon; Lee, Y.

In: Journal of Microbiology and Biotechnology, Vol. 10, No. 5, 01.01.2000, p. 595-598.

Research output: Contribution to journalArticle

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