A direct sandwich ELISA to detect antibodies against the C-terminal region of merozoite surface protein 1 could be a useful diagnostic method to identify Plasmodium vivax exposed persons

Kook Lim, Jae Park, Mi Sohn, Seungwon Lee, Jae Oh, Hyung Kim, Young Bahk, Yu Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We expressed a C-terminal 108-aa region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1c) excluding the C-end transmembrane region in Escherichia coli in order to evaluate the antibody level to MSP in Korean malaria patients. We optimized a direct sandwich enzyme-linked immunosorbent assay (ELISA) method to simultaneously determine the total antibody levels, including IgG and IgM, to PvMSP1c. If the cut-off for seropositivity was determined as the mean+3SD of the antibody levels of the negative control group, the antibody levels were positive in 99.5% of the patient group (sensitivity 199/200). The antibody levels were negative in 99.4% of the negative control group (specificity 504/507). The positive reactions in the negative control group came from non-specific reactions, as confirmed by a competition assay. This direct sandwich ELISA for PvMSP1c antibody could prove to be a useful tool for the diagnosis of malaria patients and for blood screening in blood banks.

Original languageEnglish
Pages (from-to)855-860
Number of pages6
JournalParasitology Research
Volume88
Issue number9
DOIs
Publication statusPublished - 2002 Sep 20

Fingerprint

Merozoite Surface Protein 1
Plasmodium vivax
merozoites
surface proteins
sandwiches
diagnostic techniques
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antibodies
Antibodies
malaria
Control Groups
Malaria
Blood Banks
blood
seroprevalence
Immunoglobulin M
Immunoglobulin G
Escherichia coli
screening

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

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abstract = "We expressed a C-terminal 108-aa region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1c) excluding the C-end transmembrane region in Escherichia coli in order to evaluate the antibody level to MSP in Korean malaria patients. We optimized a direct sandwich enzyme-linked immunosorbent assay (ELISA) method to simultaneously determine the total antibody levels, including IgG and IgM, to PvMSP1c. If the cut-off for seropositivity was determined as the mean+3SD of the antibody levels of the negative control group, the antibody levels were positive in 99.5{\%} of the patient group (sensitivity 199/200). The antibody levels were negative in 99.4{\%} of the negative control group (specificity 504/507). The positive reactions in the negative control group came from non-specific reactions, as confirmed by a competition assay. This direct sandwich ELISA for PvMSP1c antibody could prove to be a useful tool for the diagnosis of malaria patients and for blood screening in blood banks.",
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A direct sandwich ELISA to detect antibodies against the C-terminal region of merozoite surface protein 1 could be a useful diagnostic method to identify Plasmodium vivax exposed persons. / Lim, Kook; Park, Jae; Sohn, Mi; Lee, Seungwon; Oh, Jae; Kim, Hyung; Bahk, Young; Kim, Yu.

In: Parasitology Research, Vol. 88, No. 9, 20.09.2002, p. 855-860.

Research output: Contribution to journalArticle

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AU - Oh, Jae

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AU - Bahk, Young

AU - Kim, Yu

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