A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.
|Number of pages||4|
|Journal||Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)|
|Publication status||Published - 2002|
All Science Journal Classification (ASJC) codes
- Mechanical Engineering
- Electrical and Electronic Engineering
- Control and Systems Engineering