Abstract
A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.
| Original language | English |
|---|---|
| Pages (from-to) | 133-136 |
| Number of pages | 4 |
| Journal | Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS) |
| DOIs | |
| Publication status | Published - 2002 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Mechanical Engineering
- Electrical and Electronic Engineering
- Control and Systems Engineering
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}
A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay. / Kim, Joon Ho; Kim, Byoung Gyun; Nam, Hyukjun; Park, Dae Eun; Yun, Kwang Seok; Yoon, Jun Bo; You, Jichang; Yoon, Euisik.
In: Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), 01.01.2002, p. 133-136.Research output: Contribution to journal › Article
TY - JOUR
T1 - A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay
AU - Kim, Joon Ho
AU - Kim, Byoung Gyun
AU - Nam, Hyukjun
AU - Park, Dae Eun
AU - Yun, Kwang Seok
AU - Yoon, Jun Bo
AU - You, Jichang
AU - Yoon, Euisik
PY - 2002/1/1
Y1 - 2002/1/1
N2 - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.
AB - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.
UR - http://www.scopus.com/inward/record.url?scp=0036198841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036198841&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2002.984222
DO - 10.1109/MEMSYS.2002.984222
M3 - Article
AN - SCOPUS:0036198841
SP - 133
EP - 136
JO - Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
JF - Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
SN - 1084-6999
ER -