A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

Joon Ho Kim; Byoung Gyun Kim; Hyukjun Nam; Dae Eun Park; Kwang Seok Yun; Jun Bo Yoon; Jichang You; Euisik Yoon

doi:10.1109/MEMSYS.2002.984222

A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

Joon Ho Kim, Byoung Gyun Kim, Hyukjun Nam, Dae Eun Park, Kwang Seok Yun, Jun Bo Yoon, Jichang You, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm² and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

Original language	English
Pages (from-to)	133-136
Number of pages	4
Journal	Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2002.984222
Publication status	Published - 2002

All Science Journal Classification (ASJC) codes

Mechanical Engineering
Electrical and Electronic Engineering
Control and Systems Engineering

Access to Document

10.1109/MEMSYS.2002.984222

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@article{e21610df32914fa08500086a0f6cde70,

title = "A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay",

abstract = "A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.",

author = "Kim, {Joon Ho} and Kim, {Byoung Gyun} and Hyukjun Nam and Park, {Dae Eun} and Yun, {Kwang Seok} and Yoon, {Jun Bo} and Jichang You and Euisik Yoon",

year = "2002",

doi = "10.1109/MEMSYS.2002.984222",

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T1 - A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

AU - Kim, Joon Ho

AU - Kim, Byoung Gyun

AU - Nam, Hyukjun

AU - Park, Dae Eun

AU - Yun, Kwang Seok

AU - Yoon, Jun Bo

AU - You, Jichang

AU - Yoon, Euisik

PY - 2002

Y1 - 2002

N2 - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

AB - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

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