A Distinct Role for Interleukin-6 as a Major Mediator of Cellular Adjustment to an Altered Culture Condition

Hwa Kyung Son, Iha Park, Jue Young Kim, Do Kyeong Kim, Rasika P. Illeperuma, Jung Yoon Bae, Doo Young Lee, Eun Sang Oh, Da Woon Jung, Darren R. Williams, Jin Kim

Research output: Contribution to journalArticle

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Abstract

Tissue microenvironment adjusts biological properties of different cells by modulating signaling pathways and cell to cell interactions. This study showed that epithelial-mesenchymal transition (EMT)/ mesenchymal-epithelial transition (MET) can be modulated by altering culture conditions. HPV E6/E7-transfected immortalized oral keratinocytes (IHOK) cultured in different media displayed reversible EMT/MET accompanied by changes in cell phenotype, proliferation, gene expression at transcriptional, and translational level, and migratory and invasive activities. Cholera toxin, a major supplement to culture medium, was responsible for inducing the morphological and biological changes of IHOK. Cholera toxin per se induced EMT by triggering the secretion of interleukin 6 (IL-6) from IHOK. We found IL-6 to be a central molecule that modulates the reversibility of EMT based not only on the mRNA level but also on the level of secretion. Taken together, our results demonstrate that IL-6, a cytokine whose transcription is activated by alterations in culture conditions, is a key molecule for regulating reversible EMT/MET. This study will contribute to understand one way of cellular adjustment for surviving in unfamiliar conditions. J. Cell. Biochem. 116: 2552-2562, 2015.

Original languageEnglish
Pages (from-to)2552-2562
Number of pages11
JournalJournal of Cellular Biochemistry
Volume116
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1

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Epithelial-Mesenchymal Transition
Interleukin-6
Cholera Toxin
Molecules
Cell proliferation
Transcription
Gene expression
Culture Media
Tissue
Cytokines
Messenger RNA
Keratinocytes
Cell Communication
Cell Proliferation
Phenotype
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Son, Hwa Kyung ; Park, Iha ; Kim, Jue Young ; Kim, Do Kyeong ; Illeperuma, Rasika P. ; Bae, Jung Yoon ; Lee, Doo Young ; Oh, Eun Sang ; Jung, Da Woon ; Williams, Darren R. ; Kim, Jin. / A Distinct Role for Interleukin-6 as a Major Mediator of Cellular Adjustment to an Altered Culture Condition. In: Journal of Cellular Biochemistry. 2015 ; Vol. 116, No. 11. pp. 2552-2562.
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Son, HK, Park, I, Kim, JY, Kim, DK, Illeperuma, RP, Bae, JY, Lee, DY, Oh, ES, Jung, DW, Williams, DR & Kim, J 2015, 'A Distinct Role for Interleukin-6 as a Major Mediator of Cellular Adjustment to an Altered Culture Condition', Journal of Cellular Biochemistry, vol. 116, no. 11, pp. 2552-2562. https://doi.org/10.1002/jcb.25200

A Distinct Role for Interleukin-6 as a Major Mediator of Cellular Adjustment to an Altered Culture Condition. / Son, Hwa Kyung; Park, Iha; Kim, Jue Young; Kim, Do Kyeong; Illeperuma, Rasika P.; Bae, Jung Yoon; Lee, Doo Young; Oh, Eun Sang; Jung, Da Woon; Williams, Darren R.; Kim, Jin.

In: Journal of Cellular Biochemistry, Vol. 116, No. 11, 01.11.2015, p. 2552-2562.

Research output: Contribution to journalArticle

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