The xeroderma pigmentosum complementation group F (XPF) protein is a structure-specific endonuclease in a complex with ERCC1 and is essential for nucleotide excision repair (NER). We report a single cDNA of Caenorhabditis elegans (C. elegans) encoding highly similar protein to human XPF and other XPF members. We propose to name the corresponding C. elegans gene xpf. Messenger RNA for C. elegans xpf is 5′-tagged with a SL2 splice leader, suggesting an operon-like expression for xpf. Using RNAi, we showed that loss of C. elegans xpf function caused hypersensitivity to ultra-violet (UV) irradiation, as observed in enhanced germ cell apoptosis and increased embryonic lethality. This study suggests that C. elegans xpf is conserved in evolution and plays a role in the repair of UV-damaged DNA in C. elegans.
Bibliographical noteFunding Information:
We thank Dr. Yuji Kohara (National institute of Genetics, Japan) for EST clones, Dr. Alan Coulson (Sanger Center) for the C47D12 clone, and Dr. Andrew Fire (Carnegie Institute) for the plasmid pPD129.36 and E. coli HT115. The N2 C. elegans strain was obtained from C. elegans Genetic Center (St. Paul, MN, USA), which is supported by the National Center for Research Resources. This work was supported by the Basic Research Program of the Korea Science and Engineering Foundation grant (R01-2002-00361-0) to B. Ahn and D. Suh, and by University of Ulsan research grant to B. Ahn.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology