A DNA vaccine encoding a fatty acid-binding protein of Clonorchis sinensis induces protective immune response in sprague-dawley rats

J. S. Lee, I. S. Kim, W. M. Sohn, J. Lee, T. S. Yong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Clonorchis sinensis, the Chinese liver fluke, resides chronically in the biliary tract, and fatty acid-binding protein (FABP) is known to play an important role in the intracellular transport of long-chain fatty acids obtained from the host. Although FABP has stimulated considerable interest as a vaccine candidate, the nature of C. sinensis FABP (CsFABP) remains unclear. We investigated the immunogenicity and protective efficacy of a DNA vaccine encoding CsFABP. The intradermal injection of plasmid DNA carrying the CsFABP gene (pcDNA3.1-FABP) into Sprague-Dawley (SD) rats induced both humoural and cellular immune responses. Animals injected with pcDNA3.1-FABP developed FABP-specific antibody, which is dominance of IgG2a in sera. In addition, the DNA vaccine elicited the production of IFN-γ, but not the production of IL-4 in spleen cells stimulated with recombinant FABP. Moreover, pcDNA3.1-FABP induced a significant level of protection, decreased worm burden (40.9%, P < 0.05) in SD rats against C. sinensis metacerariae challenge. These results suggest that pcDNA3.1-FABP induces a typical T helper-1-dominated immune response and it is a good candidate for use in future clonorchiasis vaccination studies.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalScandinavian Journal of Immunology
Volume63
Issue number3
DOIs
Publication statusPublished - 2006 Mar

All Science Journal Classification (ASJC) codes

  • Immunology

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