A fluorescent polymer for patterning of mesenchymal stem cells

Jungmok You, June Seok Heo, Jiyea Lee, Han Soo Kim, Hyun Ok Kim, Eunkyoung Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

UV exposure of a fluorescent polymer, diphenylamino-s-triazine bridged p-phenylene vinylene polymer (DTOPV), resulted in fluorescence quenching and a change in surface wettability via photo-oxidation. Patterned polymer films were prepared simply by exposing the polymer film to UV source through a photomask under air. The UV-exposed region was highly biocompatible and provided selective mesenchymal stem cells (MSCs) attachment on it. This allowed cell alignment and patterning along the line patterns of linear, curved, and even various letter shapes. The proliferation rate of MSCs cultured on UV exposed surface (DTOPV+UV) was higher than that of the unexposed surface, and the cells were increased to10-fold after 6 days. The attachment of MSCs was highly selective to the UV-exposed pattern in the presence of collagen and gelatin, which induced cell patterning and attachment through hydrophilic interaction with the UV exposed area. Taking advantage of the emission from the DTOPV pattern, the cell location and pattern images were easily detected through a microscope with or without an excitation probe beam. These studies provide an exciting opportunity for novel cell patterning by a simple photopatterning process using a highly fluorescent DTOPV.

Original languageEnglish
Pages (from-to)3326-3332
Number of pages7
JournalMacromolecules
Volume42
Issue number9
DOIs
Publication statusPublished - 2009 May 12

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

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    You, J., Heo, J. S., Lee, J., Kim, H. S., Kim, H. O., & Kim, E. (2009). A fluorescent polymer for patterning of mesenchymal stem cells. Macromolecules, 42(9), 3326-3332. https://doi.org/10.1021/ma802722q