A Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markers

Kyung Soo Park, Seung Won Shin, Min Su Jang, Woojung Shin, Kisuk Yang, Junhong Min, Seung Woo Cho, Byung Keun Oh, Jong Wook Bae, Sunghwan Jung, Jeong Woo Choi, Soong Ho Um

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Accurate cancer diagnosis often requires extraction and purification of genetic materials from cells, and sophisticated instrumentations that follow. Otherwise in order to directly treat the diagnostic materials to cells, multiple steps to optimize dose concentration and treatment time are necessary due to diversity in cellular behaviors. These processes may offer high precision but hinder fast analysis of cancer, especially in clinical situations that need rapid detection and characterization of cancer. Here we present a novel fluorescent tile DNA nanostructure delivered to cancer cytosol by employing nanoparticle technology. Its structural anisotropicity offers easy manipulation for multifunctionalities, enabling the novel DNA nanostructure to detect intracellular cancer RNA markers with high specificity within 30 minutes post treatment, while the nanoparticle property bypasses the requirement of treatment optimization, effectively reducing the complexity of applying the system for cancer diagnosis. Altogether, the system offers a precise and rapid detection of cancer, suggesting the future use in the clinical fields.

Original languageEnglish
Article number18497
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 18

Bibliographical note

Funding Information:
We especially thank Mr. Woo Chul Song for his ardent help and discussion. This work was supported by grants from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant no. HI14C3301) and by Basic Science Research Programs through the National Research Foundation (NRF) funded by the Ministry of Science ICT and Future Planning (Grant Nos. 2013R1A1A1058670 and 2013R1A1A2016781) and by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP) (2013K1A4A3055268).

Publisher Copyright:
© 2015, Nature Publishing Group. All rights reserved.

All Science Journal Classification (ASJC) codes

  • General

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