A gene and neural stem cell therapy platform based on neuronal cell type–inducible gene overexpression

Jinsoo Oh, Youngsang You, Yeomin Yun, Hye Lan Lee, Do Heum Yoon, Minhyung Lee, Yoon Ha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type‒inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type‒inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. Results: NSCs introduced via the neuronal cell type‒inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. Conclusion: We demonstrated that a neuronal cell type‒inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI.

Original languageEnglish
Pages (from-to)1036-1043
Number of pages8
JournalYonsei medical journal
Volume56
Issue number4
DOIs
Publication statusPublished - 2015 Jul 1

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Neural Stem Cells
Cell- and Tissue-Based Therapy
Luciferases
Phosphopyruvate Hydratase
Genes
Spinal Cord Injuries
Therapeutics
Therapeutic Uses
Transgenes
Neurodegenerative Diseases
Gene Expression
Neurons

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Oh, Jinsoo ; You, Youngsang ; Yun, Yeomin ; Lee, Hye Lan ; Yoon, Do Heum ; Lee, Minhyung ; Ha, Yoon. / A gene and neural stem cell therapy platform based on neuronal cell type–inducible gene overexpression. In: Yonsei medical journal. 2015 ; Vol. 56, No. 4. pp. 1036-1043.
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abstract = "Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type‒inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type‒inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. Results: NSCs introduced via the neuronal cell type‒inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. Conclusion: We demonstrated that a neuronal cell type‒inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI.",
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A gene and neural stem cell therapy platform based on neuronal cell type–inducible gene overexpression. / Oh, Jinsoo; You, Youngsang; Yun, Yeomin; Lee, Hye Lan; Yoon, Do Heum; Lee, Minhyung; Ha, Yoon.

In: Yonsei medical journal, Vol. 56, No. 4, 01.07.2015, p. 1036-1043.

Research output: Contribution to journalArticle

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T1 - A gene and neural stem cell therapy platform based on neuronal cell type–inducible gene overexpression

AU - Oh, Jinsoo

AU - You, Youngsang

AU - Yun, Yeomin

AU - Lee, Hye Lan

AU - Yoon, Do Heum

AU - Lee, Minhyung

AU - Ha, Yoon

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N2 - Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type‒inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type‒inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. Results: NSCs introduced via the neuronal cell type‒inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. Conclusion: We demonstrated that a neuronal cell type‒inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI.

AB - Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type‒inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type‒inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. Results: NSCs introduced via the neuronal cell type‒inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. Conclusion: We demonstrated that a neuronal cell type‒inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI.

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