A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system

Sun Ju Bae, Woo Chul Song, Sung Hwan Jung, Seung Woo Cho, Dong Ik Kim, Soong Ho Um

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.

Original languageEnglish
Pages (from-to)17036-17042
Number of pages7
JournalLangmuir
Volume28
Issue number49
DOIs
Publication statusPublished - 2012 Dec 11

Fingerprint

Lipid bilayers
genes
lipids
Gels
Genes
Green Fluorescent Proteins
gels
Proteins
nuclei
DNA
proteins
matrices
deoxyribonucleic acid
Gene encoding
ribonucleic acids
Scaffolds (biology)
Nanotechnology
Scaffolds
plasmids
Lithography

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Bae, S. J., Song, W. C., Jung, S. H., Cho, S. W., Kim, D. I., & Um, S. H. (2012). A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system. Langmuir, 28(49), 17036-17042. https://doi.org/10.1021/la303498k
Bae, Sun Ju ; Song, Woo Chul ; Jung, Sung Hwan ; Cho, Seung Woo ; Kim, Dong Ik ; Um, Soong Ho. / A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system. In: Langmuir. 2012 ; Vol. 28, No. 49. pp. 17036-17042.
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Bae, SJ, Song, WC, Jung, SH, Cho, SW, Kim, DI & Um, SH 2012, 'A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system', Langmuir, vol. 28, no. 49, pp. 17036-17042. https://doi.org/10.1021/la303498k

A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system. / Bae, Sun Ju; Song, Woo Chul; Jung, Sung Hwan; Cho, Seung Woo; Kim, Dong Ik; Um, Soong Ho.

In: Langmuir, Vol. 28, No. 49, 11.12.2012, p. 17036-17042.

Research output: Contribution to journalArticle

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