A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system

Sun Ju Bae, Woo Chul Song, Sung Hwan Jung, Seung Woo Cho, Dong Ik Kim, Soong Ho Um

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.

Original languageEnglish
Pages (from-to)17036-17042
Number of pages7
JournalLangmuir
Volume28
Issue number49
DOIs
Publication statusPublished - 2012 Dec 11

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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