A graphical method of determining the Michaelis-Menten constant free of external mass transfer resistance for immobilized enzymes in a packed bed reactor

Baik L. Seong; Moon H. Han; Jong M. Park; Cha Y. Choi

doi:10.1007/BF00158687

A graphical method of determining the Michaelis-Menten constant free of external mass transfer resistance for immobilized enzymes in a packed bed reactor

Baik L. Seong, Moon H. Han, Jong M. Park, Cha Y. Choi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent K_m value vs. the reciprocal of superficial velocity in column allowed an easy calculation of K_m free of external mass transfer resistance. An asymptotic value of apparent K_m value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient k_L, approached a definite value at this condition.

Original language	English
Pages (from-to)	607-612
Number of pages	6
Journal	Biotechnology Letters
Volume	3
Issue number	11
DOIs	https://doi.org/10.1007/BF00158687
Publication status	Published - 1981 Nov

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF00158687

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abstract = "A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent Km value vs. the reciprocal of superficial velocity in column allowed an easy calculation of Km free of external mass transfer resistance. An asymptotic value of apparent Km value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient kL, approached a definite value at this condition.",

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AU - Seong, Baik L.

AU - Han, Moon H.

AU - Park, Jong M.

AU - Choi, Cha Y.

PY - 1981/11

Y1 - 1981/11

N2 - A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent Km value vs. the reciprocal of superficial velocity in column allowed an easy calculation of Km free of external mass transfer resistance. An asymptotic value of apparent Km value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient kL, approached a definite value at this condition.

AB - A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent Km value vs. the reciprocal of superficial velocity in column allowed an easy calculation of Km free of external mass transfer resistance. An asymptotic value of apparent Km value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient kL, approached a definite value at this condition.

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A graphical method of determining the Michaelis-Menten constant free of external mass transfer resistance for immobilized enzymes in a packed bed reactor

Abstract

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