A graphical method of determining the Michaelis-Menten constant free of external mass transfer resistance for immobilized enzymes in a packed bed reactor

Baik L. Seong, Moon H. Han, Jong M. Park, Cha Y. Choi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data points in the plot of apparent Km value vs. the reciprocal of superficial velocity in column allowed an easy calculation of Km free of external mass transfer resistance. An asymptotic value of apparent Km value at infinite zero superficial velocity was ascribed to the fact that the mass transfer coefficient kL, approached a definite value at this condition.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalBiotechnology Letters
Volume3
Issue number11
DOIs
Publication statusPublished - 1981 Nov

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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