A herpes simplex virus-1 vector containing the rat tyrosine hydroxylase promoter directs cell type-specific expression of beta-galactosidase in cultured rat peripheral neurons

Young Jun Oh, Mark Moffat, Shou Wong, Donna Ullrey, Alfred I. Geller, Karen L. O'Malley

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A defective herpes simplex virus-1 (HSV-1) vector system was used to study cell type-specific expression of the tyrosine hydroxylase (TH) gene. HSV-1 particles containing 663 bp (pTHlac 663), 278 bp (pTHlac 278), or 181 bp (pTHlac 181) of the rat TH promoter driving E. coli LacZ were used to infect superior cervical ganglia (SCG: TH-expressing tissue) and dorsal root ganglia (DRG: non-TH-expressing tissue) cultures. One day after infection, expression of β-galactosidase was visualized by X-gal cytochemistry. Following viral transduction with pTHlac 663 at a multiplicity of infection of 0.2, 14.4% of the SCG neurons were X-gal positive whereas only about 0.9% of DRG neurons were X-gal positive. Infection with either pTHlac 278 or 181 resulted in 3-fold more X-gal-positive DRG neurons. These results suggest that (i) the defective HSV-1 vector system may be useful in defining regulatory promoter motifs; (ii) 663 bp of the rat TH promoter contains sufficient information for cell type-specific expression in peripheral nervous system neurons; and (iii) sequences between -278 and -663 contain an element(s) that represses gene expression in non-catecholamingeric neurons.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalMolecular Brain Research
Volume35
Issue number1-2
DOIs
Publication statusPublished - 1996 Jan 1

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Human Herpesvirus 1
Tyrosine 3-Monooxygenase
beta-Galactosidase
Diagnosis-Related Groups
Neurons
Defective Viruses
Infection
Galactosidases
Superior Cervical Ganglion
Histocytochemistry
Peripheral Nervous System
Spinal Ganglia
Mixed Function Oxygenases
Virion
Escherichia coli
Gene Expression
5-bromo-4-chloro-3-indolyl beta-galactoside
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

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title = "A herpes simplex virus-1 vector containing the rat tyrosine hydroxylase promoter directs cell type-specific expression of beta-galactosidase in cultured rat peripheral neurons",
abstract = "A defective herpes simplex virus-1 (HSV-1) vector system was used to study cell type-specific expression of the tyrosine hydroxylase (TH) gene. HSV-1 particles containing 663 bp (pTHlac 663), 278 bp (pTHlac 278), or 181 bp (pTHlac 181) of the rat TH promoter driving E. coli LacZ were used to infect superior cervical ganglia (SCG: TH-expressing tissue) and dorsal root ganglia (DRG: non-TH-expressing tissue) cultures. One day after infection, expression of β-galactosidase was visualized by X-gal cytochemistry. Following viral transduction with pTHlac 663 at a multiplicity of infection of 0.2, 14.4{\%} of the SCG neurons were X-gal positive whereas only about 0.9{\%} of DRG neurons were X-gal positive. Infection with either pTHlac 278 or 181 resulted in 3-fold more X-gal-positive DRG neurons. These results suggest that (i) the defective HSV-1 vector system may be useful in defining regulatory promoter motifs; (ii) 663 bp of the rat TH promoter contains sufficient information for cell type-specific expression in peripheral nervous system neurons; and (iii) sequences between -278 and -663 contain an element(s) that represses gene expression in non-catecholamingeric neurons.",
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A herpes simplex virus-1 vector containing the rat tyrosine hydroxylase promoter directs cell type-specific expression of beta-galactosidase in cultured rat peripheral neurons. / Oh, Young Jun; Moffat, Mark; Wong, Shou; Ullrey, Donna; Geller, Alfred I.; O'Malley, Karen L.

In: Molecular Brain Research, Vol. 35, No. 1-2, 01.01.1996, p. 227-236.

Research output: Contribution to journalArticle

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T1 - A herpes simplex virus-1 vector containing the rat tyrosine hydroxylase promoter directs cell type-specific expression of beta-galactosidase in cultured rat peripheral neurons

AU - Oh, Young Jun

AU - Moffat, Mark

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AU - Geller, Alfred I.

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