A high throughput micro-array system of polymer surfaces for the manipulation of primary pancreatic islet cells

Ying Mei, Jennifer Hollister-Lock, Said R. Bogatyrev, Seung Woo Cho, Gordon C. Weir, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We developed a high throughput micro-arrayed polymer system for the study of polymer surfaces for islet cell culture. A micro-arrayed library with 496 different polymers was synthesized and used to examine attachment and insulin expression of islet cells. While most polymers were not supportive, several related polymers were identified as suitable (" hit's" ). The "hit" arrays composed of "hit" polymers with 36 replicates were fabricated to confirm their capacities to support the attachment of islet cells, and these capacities were further validated in large surfaces. Notably, the attachment of islet cells on these synthetic polymeric films has been found to be as supportive as 804G supernatant coated tissue culture polystyrene dishes, one of the most extensively used substrates for the islet cell attachment. Interestingly, the polymeric surfaces optimal for a different cell type, hES derived cells, were distinct, highlighting the utility of these approaches for identifying cell type specific surfaces.

Original languageEnglish
Pages (from-to)8989-8995
Number of pages7
JournalBiomaterials
Volume31
Issue number34
DOIs
Publication statusPublished - 2010 Dec

Bibliographical note

Funding Information:
We gratefully acknowledge Ed Luther for the help with the laser scanning cytometry. Funding was kindly provided by NIH grant R01 ( DE016516-03 ) and Juvenile Diabetes Research Foundation .

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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