A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Howon Lee, Hyoki Kim, Sungsik Kim, Taehoon Ryu, Hwangbeom Kim, Duhee Bang, Sunghoon Kwon

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called â € Sniper Cloningâ €™ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

Original languageEnglish
Article number6073
JournalNature communications
Volume6
DOIs
Publication statusPublished - 2015 Feb

Fingerprint

sequencing
retrieval
deoxyribonucleic acid
platforms
Throughput
DNA
Clone Cells
Oligonucleotide Array Sequence Analysis
Technology
Cloning
Organism Cloning
Microarrays
Synthetic Biology
Bioengineering
Biological Science Disciplines
Genomics
bioengineering
DNA sequences
Lasers
Merging

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Lee, Howon ; Kim, Hyoki ; Kim, Sungsik ; Ryu, Taehoon ; Kim, Hwangbeom ; Bang, Duhee ; Kwon, Sunghoon. / A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform. In: Nature communications. 2015 ; Vol. 6.
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abstract = "Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called {\^a} € Sniper Cloning{\^a} €™ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.",
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A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform. / Lee, Howon; Kim, Hyoki; Kim, Sungsik; Ryu, Taehoon; Kim, Hwangbeom; Bang, Duhee; Kwon, Sunghoon.

In: Nature communications, Vol. 6, 6073, 02.2015.

Research output: Contribution to journalArticle

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AU - Lee, Howon

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AU - Kim, Hwangbeom

AU - Bang, Duhee

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