A highly sensitive and label free biosensing platform for wireless sensor node system

Sang Gyu Kim, Hee Jo Lee, Jung Hyun Lee, Hyo Il Jung, Jong Gwan Yook

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this paper, we propose a radio-frequency (RF) biosensor platform based on oscillation frequency deviation at 2.4. GHz. Its feasibility is experimentally demonstrated with the well-known biomolecular binding systems such as biotin-streptavidin and deoxyribonucleic acid (DNA) hybridization. For a basic principle of our biosensing system, the impedance of a resonator with the biomolecular immobilization is at first varied so that the corresponding change results in frequency change of an oscillator. Especially, to enhance the sensitivity of the proposed system, a surface acoustic wave (SAW) filter having a high-Q factor (~2000) is utilized. From the resulting component, even a small change of oscillation frequency can be transformed into a large output amplitude variation. According to the experimental results, it is found that our system shows the low detectable limit (~1. ng/ml) and fast response time (~real-time) for different target biomolecules, i.e. streptavidin and complementary DNA (cDNA). As a result, we find that our device is an effective biosensing system that can be used for a label-free and real-time measurement of the biomolecular binding events.

Original languageEnglish
Pages (from-to)362-367
Number of pages6
JournalBiosensors and Bioelectronics
Volume50
DOIs
Publication statusPublished - 2013 Dec 15

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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