A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip

Jong Min Park, Jo Il Kim, Joo Yoon Noh, Mira Kim, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.

Original languageEnglish
Pages (from-to)56-68
Number of pages13
JournalEnzyme and Microbial Technology
Volume104
DOIs
Publication statusPublished - 2017 Sep

Bibliographical note

Funding Information:
This work was supported by the Nano-Convergence Foundation [grant number: R201602210] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Industry Technology Development Program [grant number: 10063335] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea), and by the National Research Foundation of Korea [grant number: NRF-2017R1A2B4004077, NRF-2017R1A2B2004398].

Publisher Copyright:
© 2017 Elsevier Inc.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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