A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.
|Number of pages||13|
|Journal||Enzyme and Microbial Technology|
|Publication status||Published - 2017 Sept|
Bibliographical noteFunding Information:
This work was supported by the Nano-Convergence Foundation [grant number: R201602210] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Industry Technology Development Program [grant number: 10063335] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea), and by the National Research Foundation of Korea [grant number: NRF-2017R1A2B4004077, NRF-2017R1A2B2004398].
© 2017 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology