A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants

Adinarayana Andukuri, Meenakshi Kushwaha, Ajay Tambralli, Joel M. Anderson, Derrick R. Dean, Joel L. Berry, Young Doug Sohn, Young Sup Yoon, Brigitta C. Brott, Ho Wook Jun

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Current cardiovascular therapies are limited by the loss of endothelium, restenosis and thrombosis. The goal of this study was to develop a biomimetic hybrid nanomatrix that combined the unique properties of electrospun polycaprolactone (ePCL) nanofibers with self-assembled peptide amphiphiles (PAs). ePCL nanofibers have interconnected nanoporous structures, but are hampered by a lack of surface bioactivity to control cellular behavior. It has been hypothesized that PAs could self-assemble onto the surface of ePCL nanofibers and endow them with the characteristic properties of native endothelium. The PAs, which comprised hydrophobic alkyl tails attached to functional hydrophilic peptide sequences, contained enzyme-mediated degradable sites coupled to either endothelial cell-adhesive ligands (YIGSR) or polylysine (KKKKK) nitric oxide (NO) donors. Two different PAs (PA-YIGSR and PA-KKKKK) were successfully synthesized and mixed in a 90:10 (YK) ratio to obtain PA-YK. PA-YK was reacted with pure NO to develop PA-YK-NO, which was then self-assembled onto ePCL nanofibers to generate a hybrid nanomatrix, ePCL-PA-YK-NO. Uniform coating of self-assembled PA nanofibers on ePCL was confirmed by transmission electron microscopy. Successful NO release from ePCL-PA-YK-NO was observed. ePCL-YK and ePCL-PA-YK-NO showed significantly increased adhesion of human umbilical vein endothelial cells (HUVECs). ePCL-PA-YK-NO also showed significantly increased proliferation of HUVECs and reduced smooth muscle cell proliferation. ePCL-PA-YK-NO also displayed significantly reduced platelet adhesion compared with ePCL, ePCL-PA-YK and a collagen control. These results indicate that this hybrid nanomatrix has great potential application in cardiovascular implants.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Polycaprolactone
Biomimetics
Amphiphiles
Peptides
Nitric oxide
Nanofibers
Nitric Oxide
tyrosyl-isoleucyl-glycyl-seryl-arginine
Endothelial cells
polycaprolactone
Human Umbilical Vein Endothelial Cells
Endothelium
Adhesion
Behavior Control
Polylysine
Nitric Oxide Donors
Cell proliferation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Andukuri, Adinarayana ; Kushwaha, Meenakshi ; Tambralli, Ajay ; Anderson, Joel M. ; Dean, Derrick R. ; Berry, Joel L. ; Sohn, Young Doug ; Yoon, Young Sup ; Brott, Brigitta C. ; Jun, Ho Wook. / A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 1. pp. 225-233.
@article{9e55af25de9d48feb3098467f6805a9d,
title = "A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants",
abstract = "Current cardiovascular therapies are limited by the loss of endothelium, restenosis and thrombosis. The goal of this study was to develop a biomimetic hybrid nanomatrix that combined the unique properties of electrospun polycaprolactone (ePCL) nanofibers with self-assembled peptide amphiphiles (PAs). ePCL nanofibers have interconnected nanoporous structures, but are hampered by a lack of surface bioactivity to control cellular behavior. It has been hypothesized that PAs could self-assemble onto the surface of ePCL nanofibers and endow them with the characteristic properties of native endothelium. The PAs, which comprised hydrophobic alkyl tails attached to functional hydrophilic peptide sequences, contained enzyme-mediated degradable sites coupled to either endothelial cell-adhesive ligands (YIGSR) or polylysine (KKKKK) nitric oxide (NO) donors. Two different PAs (PA-YIGSR and PA-KKKKK) were successfully synthesized and mixed in a 90:10 (YK) ratio to obtain PA-YK. PA-YK was reacted with pure NO to develop PA-YK-NO, which was then self-assembled onto ePCL nanofibers to generate a hybrid nanomatrix, ePCL-PA-YK-NO. Uniform coating of self-assembled PA nanofibers on ePCL was confirmed by transmission electron microscopy. Successful NO release from ePCL-PA-YK-NO was observed. ePCL-YK and ePCL-PA-YK-NO showed significantly increased adhesion of human umbilical vein endothelial cells (HUVECs). ePCL-PA-YK-NO also showed significantly increased proliferation of HUVECs and reduced smooth muscle cell proliferation. ePCL-PA-YK-NO also displayed significantly reduced platelet adhesion compared with ePCL, ePCL-PA-YK and a collagen control. These results indicate that this hybrid nanomatrix has great potential application in cardiovascular implants.",
author = "Adinarayana Andukuri and Meenakshi Kushwaha and Ajay Tambralli and Anderson, {Joel M.} and Dean, {Derrick R.} and Berry, {Joel L.} and Sohn, {Young Doug} and Yoon, {Young Sup} and Brott, {Brigitta C.} and Jun, {Ho Wook}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.actbio.2010.08.013",
language = "English",
volume = "7",
pages = "225--233",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "1",

}

Andukuri, A, Kushwaha, M, Tambralli, A, Anderson, JM, Dean, DR, Berry, JL, Sohn, YD, Yoon, YS, Brott, BC & Jun, HW 2011, 'A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants', Acta Biomaterialia, vol. 7, no. 1, pp. 225-233. https://doi.org/10.1016/j.actbio.2010.08.013

A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants. / Andukuri, Adinarayana; Kushwaha, Meenakshi; Tambralli, Ajay; Anderson, Joel M.; Dean, Derrick R.; Berry, Joel L.; Sohn, Young Doug; Yoon, Young Sup; Brott, Brigitta C.; Jun, Ho Wook.

In: Acta Biomaterialia, Vol. 7, No. 1, 01.01.2011, p. 225-233.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants

AU - Andukuri, Adinarayana

AU - Kushwaha, Meenakshi

AU - Tambralli, Ajay

AU - Anderson, Joel M.

AU - Dean, Derrick R.

AU - Berry, Joel L.

AU - Sohn, Young Doug

AU - Yoon, Young Sup

AU - Brott, Brigitta C.

AU - Jun, Ho Wook

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Current cardiovascular therapies are limited by the loss of endothelium, restenosis and thrombosis. The goal of this study was to develop a biomimetic hybrid nanomatrix that combined the unique properties of electrospun polycaprolactone (ePCL) nanofibers with self-assembled peptide amphiphiles (PAs). ePCL nanofibers have interconnected nanoporous structures, but are hampered by a lack of surface bioactivity to control cellular behavior. It has been hypothesized that PAs could self-assemble onto the surface of ePCL nanofibers and endow them with the characteristic properties of native endothelium. The PAs, which comprised hydrophobic alkyl tails attached to functional hydrophilic peptide sequences, contained enzyme-mediated degradable sites coupled to either endothelial cell-adhesive ligands (YIGSR) or polylysine (KKKKK) nitric oxide (NO) donors. Two different PAs (PA-YIGSR and PA-KKKKK) were successfully synthesized and mixed in a 90:10 (YK) ratio to obtain PA-YK. PA-YK was reacted with pure NO to develop PA-YK-NO, which was then self-assembled onto ePCL nanofibers to generate a hybrid nanomatrix, ePCL-PA-YK-NO. Uniform coating of self-assembled PA nanofibers on ePCL was confirmed by transmission electron microscopy. Successful NO release from ePCL-PA-YK-NO was observed. ePCL-YK and ePCL-PA-YK-NO showed significantly increased adhesion of human umbilical vein endothelial cells (HUVECs). ePCL-PA-YK-NO also showed significantly increased proliferation of HUVECs and reduced smooth muscle cell proliferation. ePCL-PA-YK-NO also displayed significantly reduced platelet adhesion compared with ePCL, ePCL-PA-YK and a collagen control. These results indicate that this hybrid nanomatrix has great potential application in cardiovascular implants.

AB - Current cardiovascular therapies are limited by the loss of endothelium, restenosis and thrombosis. The goal of this study was to develop a biomimetic hybrid nanomatrix that combined the unique properties of electrospun polycaprolactone (ePCL) nanofibers with self-assembled peptide amphiphiles (PAs). ePCL nanofibers have interconnected nanoporous structures, but are hampered by a lack of surface bioactivity to control cellular behavior. It has been hypothesized that PAs could self-assemble onto the surface of ePCL nanofibers and endow them with the characteristic properties of native endothelium. The PAs, which comprised hydrophobic alkyl tails attached to functional hydrophilic peptide sequences, contained enzyme-mediated degradable sites coupled to either endothelial cell-adhesive ligands (YIGSR) or polylysine (KKKKK) nitric oxide (NO) donors. Two different PAs (PA-YIGSR and PA-KKKKK) were successfully synthesized and mixed in a 90:10 (YK) ratio to obtain PA-YK. PA-YK was reacted with pure NO to develop PA-YK-NO, which was then self-assembled onto ePCL nanofibers to generate a hybrid nanomatrix, ePCL-PA-YK-NO. Uniform coating of self-assembled PA nanofibers on ePCL was confirmed by transmission electron microscopy. Successful NO release from ePCL-PA-YK-NO was observed. ePCL-YK and ePCL-PA-YK-NO showed significantly increased adhesion of human umbilical vein endothelial cells (HUVECs). ePCL-PA-YK-NO also showed significantly increased proliferation of HUVECs and reduced smooth muscle cell proliferation. ePCL-PA-YK-NO also displayed significantly reduced platelet adhesion compared with ePCL, ePCL-PA-YK and a collagen control. These results indicate that this hybrid nanomatrix has great potential application in cardiovascular implants.

UR - http://www.scopus.com/inward/record.url?scp=78049417903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049417903&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.08.013

DO - 10.1016/j.actbio.2010.08.013

M3 - Article

C2 - 20728588

AN - SCOPUS:78049417903

VL - 7

SP - 225

EP - 233

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 1

ER -