A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains

Do Hoon Kim, Ji Hong Bong, Gu Yoo, Seo Yoon Chang, Young Wook Chang, Min Jung Kang, Joachim Jose, Jae Chul Pyun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Escherichia coli cells with autodisplayed Z-domains have been used for immunoassays of specific target analytes. In this study, a magnetite suspension was used for the washing step in immunoassays of E. coli cells with autodisplayed Z-domains. This approach enhanced the washing conditions for these immunoassays by determining (1) the optimal concentration of the magnetite suspension, (2) the capacity of the magnetite suspension-based washing method to recover E. coli cells, and (3) the level at which the activity of autodisplayed Z-domains is maintained. In immunoassays of C-reactive protein (CRP), the immunoassay incorporating the magnetite suspension-based washing method showed a sensitivity and limit of detection considerably higher than those of the conventional centrifugation-based washing method. The results indicated that immunoassays incorporating the magnetite suspension-based washing method are effective for medical diagnoses based on CRP assay.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalEnzyme and Microbial Technology
Volume92
DOIs
Publication statusPublished - 2016 Oct 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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