A mechanism for the action of the compound DA-6034 on NF-κB pathway activation in helicobacter pylori-infected gastric epithelial cells

S. H. Ko, D. Y. Yoo, Y. J. Kim, S. M. Choi, K. K. Kang, H. Kim, N. Kim, J. S. Kim, J. M. Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)


DA-6034 is a synthetic derivative of eupatilin, a flavonoid with anti-inflammatory effects. The aim of this study was to investigate the effects of DA-6034 on the interactions between IκB kinase (IKK) and heat shock protein 90 (Hsp90), and activation of the nuclear factor-kappaB (NF-κB) signalling pathway in human gastric epithelial cells infected with Helicobacter pylori. MKN-45 gastric epithelial cell line was treated with DA-6034 and H. pylori. DA-6034 significantly inhibited NF-κB activation and upregulated the expressions of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 in MKN-45 cells infected with H. pylori. However, DA-6034 did not influence activator protein-1 DNA binding activity in H. pylori-infected gastric epithelial cells. Pretreatment with DA-6034 attenuated the H. pylori-induced increase in IKK activity, and Hsp90 was associated with IKK-α and IKK-γ in MKN-45 cells. Treatment with DA-6034 dissociated the Hsp90 and IKK-γ complex in H. pylori-infected cells, leading to the inhibition of IL-8 expression. These results suggest that the eupatilin derivative 7-carboxymethyloxy-3',4',5-trimethoxy flavone has anti-inflammatory activity in gastric epithelial cells infected with H. pylori through the promotion of the dissociation of the IKK-γ-Hsp90 complex and suppression of NF-κB signalling.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalScandinavian Journal of Immunology
Issue number3
Publication statusPublished - 2011 Sep 1


All Science Journal Classification (ASJC) codes

  • Immunology

Cite this