A melting method for RNA extraction from the mucosal membrane of the mouse middle ear

Young Joon Seo, Sung Huhn Kim, In Seok Moon, Jae Young Choi

Research output: Contribution to journalArticle

Abstract

Purpose: There is much confusion surrounding the methods of RNA extraction from the middle ear mucosa of mice. In this study, we worked to develop a “melting method,” which is faster, purer, and more reliable than other methods in common use. Materials and Methods: Thirty-two ears were used for this study. Light microscopy with hematoxylin-eosin staining of the bullae, scanning electron microscopy (SEM), spectrophotometer analysis, and reverse transcription polymerase chain reaction were performed before and after melting the half lateral bullae, which were detached from the temporal bone by using a lateral retroauricular approach. Results: Each resected half bulla contained a well distributed mucosal membrane. After a TRIzol melting duration of 10‒30 minutes, only mucosal marker (MUC5AC) was expressed without bony marker (total osteocalcin). The same results were determined from SEM. Conclusion: This melting method, compared with stripping and irrigation methods, is effective and offers an easier, more robust approach to extracting RNA from the middle ear mucosal membranes of mice.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalYonsei medical journal
Volume56
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1

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Middle Ear
Freezing
RNA
Membranes
Blister
Electron Scanning Microscopy
Temporal Bone
Osteocalcin
Hematoxylin
Eosine Yellowish-(YS)
Reverse Transcription
Ear
Microscopy
Mucous Membrane
Staining and Labeling
Light
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Seo, Young Joon ; Kim, Sung Huhn ; Moon, In Seok ; Choi, Jae Young. / A melting method for RNA extraction from the mucosal membrane of the mouse middle ear. In: Yonsei medical journal. 2015 ; Vol. 56, No. 2. pp. 497-502.
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A melting method for RNA extraction from the mucosal membrane of the mouse middle ear. / Seo, Young Joon; Kim, Sung Huhn; Moon, In Seok; Choi, Jae Young.

In: Yonsei medical journal, Vol. 56, No. 2, 01.01.2015, p. 497-502.

Research output: Contribution to journalArticle

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