A method for electroporation to study gene function in mammary gland development

Jong Min Lee, Eun Jung Kim, Han Sung Jung

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

To reveal the specific functions of various genes during embryonic development, the manipulation of genes using techniques such as electroporation is of fundamental importance for providing direct evidence concerning function or downstream activation of signaling networks. In vitro embryo culture and electroporation are useful techniques to introduce foreign genes, for developmental biology studies. Among the various mammalian culture techniques, Trowell culture is suitable for studies of embryonic mammary gland development because of its stability and ease of use in conjunction with electroporation technique application. The manipulation of gene expression using electroporation is a useful technique for the functional analysis of a particular gene. In this protocol, full steps for electroporation and in vitro embryo culture have been described for use in embryonic mammary gland development research.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalJournal of Mammary Gland Biology and Neoplasia
Volume18
Issue number2
DOIs
Publication statusPublished - 2013 Jun 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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