To reveal the specific functions of various genes during embryonic development, the manipulation of genes using techniques such as electroporation is of fundamental importance for providing direct evidence concerning function or downstream activation of signaling networks. In vitro embryo culture and electroporation are useful techniques to introduce foreign genes, for developmental biology studies. Among the various mammalian culture techniques, Trowell culture is suitable for studies of embryonic mammary gland development because of its stability and ease of use in conjunction with electroporation technique application. The manipulation of gene expression using electroporation is a useful technique for the functional analysis of a particular gene. In this protocol, full steps for electroporation and in vitro embryo culture have been described for use in embryonic mammary gland development research.
Bibliographical noteFunding Information:
Acknowledgment This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0015661). We are thankful to Dr. KW Cho for providing in situ data.
All Science Journal Classification (ASJC) codes
- Cancer Research