We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
|Number of pages||8|
|Publication status||Published - 2013 Sep|
Bibliographical noteFunding Information:
This work was supported by grants (S.W. Cho, 2010-0020409 and 2010-0022037 ) funded by the National Research Foundation of Korea, the Ministry of Education, Science and Technology, Republic of Korea . We also acknowledge supports to S. Chung from the Ministry for Health & Welfare Affairs ( A092255 ) and the National Research Foundation of Korea ( NRF-2012M3A6A-2011-0032211 ).
All Science Journal Classification (ASJC) codes
- Ceramics and Composites
- Mechanics of Materials