A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Kisuk Yang, Sewoon Han, Yoojin Shin, Eunkyung Ko, Jin Kim, Kook In Park, Seok Chung, Seung Woo Cho

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.

Original languageEnglish
Pages (from-to)6607-6614
Number of pages8
JournalBiomaterials
Volume34
Issue number28
DOIs
Publication statusPublished - 2013 Sep

Bibliographical note

Funding Information:
This work was supported by grants (S.W. Cho, 2010-0020409 and 2010-0022037 ) funded by the National Research Foundation of Korea, the Ministry of Education, Science and Technology, Republic of Korea . We also acknowledge supports to S. Chung from the Ministry for Health & Welfare Affairs ( A092255 ) and the National Research Foundation of Korea ( NRF-2012M3A6A-2011-0032211 ).

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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